Split light up aptamers as a probing tool for nucleic acids.
Fluorescent probes for RNA
Hybridization probe
Label-free probes
Light up aptamers
Single nucleotide selectivity
Spinach aptamer
Journal
Methods (San Diego, Calif.)
ISSN: 1095-9130
Titre abrégé: Methods
Pays: United States
ID NLM: 9426302
Informations de publication
Date de publication:
01 2022
01 2022
Historique:
received:
12
03
2021
revised:
02
05
2021
accepted:
05
05
2021
pubmed:
17
5
2021
medline:
1
4
2022
entrez:
16
5
2021
Statut:
ppublish
Résumé
Aptamers that bind non-fluorescent dyes and increase their fluorescence can be converted to fluorescent sensors. Here, we discuss and provide guidance for the design of split (binary) light up aptameric sensors (SLAS) for nucleic acid analysis. SLAS consist of two RNA or DNA strands and a fluorogenic organic dye added as a buffer component. The two strands hybridize to the analyzed DNA or RNA sequence and form a dye-binding pocket, followed by dye binding, and increase in its fluorescence. SLAS can detect nucleic acids in a cost-efficient label-free format since it does not require conjugation of organic dyes with nucleic acids. SLAS design is preferable over monolith fluorescent sensors due to simpler assay optimization and improved selectivity. RNA-based SLAS can be expressed in cells and used for intracellular monitoring and imaging biological molecules.
Identifiants
pubmed: 33992774
pii: S1046-2023(21)00134-1
doi: 10.1016/j.ymeth.2021.05.008
pii:
doi:
Substances chimiques
Aptamers, Nucleotide
0
Fluorescent Dyes
0
Nucleic Acids
0
RNA
63231-63-0
DNA
9007-49-2
Types de publication
Journal Article
Langues
eng
Sous-ensembles de citation
IM
Pagination
82-88Informations de copyright
Copyright © 2021 Elsevier Inc. All rights reserved.