Evaluation of Bioactivity of Strigolactone-Related Molecules by a Quantitative Luminometer Bioassay.
Arabidopsis
/ drug effects
Arabidopsis Proteins
/ agonists
Biological Assay
Genes, Reporter
Heterocyclic Compounds, 3-Ring
/ pharmacology
Lactones
/ pharmacology
Luciferases, Firefly
/ genetics
Luminescent Measurements
Plant Growth Regulators
/ pharmacology
Plants, Genetically Modified
/ drug effects
Proteolysis
Receptors, Cell Surface
/ agonists
Recombinant Fusion Proteins
/ metabolism
Signal Transduction
Bioassay
DWARF14
Luciferase
Perception
Plant hormones
Strigolactones
Journal
Methods in molecular biology (Clifton, N.J.)
ISSN: 1940-6029
Titre abrégé: Methods Mol Biol
Pays: United States
ID NLM: 9214969
Informations de publication
Date de publication:
2021
2021
Historique:
entrez:
24
5
2021
pubmed:
25
5
2021
medline:
11
8
2021
Statut:
ppublish
Résumé
The binding of strigolactones to their receptor, the α/β hydrolase DWARF14 (D14), leads to the modulation of transcriptional activity by destabilization of specific transcriptional corepressors via proteasomal degradation. Subsequently, strigolactones also promote D14 degradation by the same pathway. Here we describe an innovative quantitative bioassay based on Arabidopsis transgenic lines expressing AtD14 fused to the firefly luciferase, developed to identify new strigolactone analogs capable to activate the strigolactone signaling.
Identifiants
pubmed: 34028688
doi: 10.1007/978-1-0716-1429-7_15
doi:
Substances chimiques
Arabidopsis Proteins
0
DWARF14 protein, Arabidopsis
0
GR24 strigolactone
0
Heterocyclic Compounds, 3-Ring
0
Lactones
0
Plant Growth Regulators
0
Receptors, Cell Surface
0
Recombinant Fusion Proteins
0
Luciferases, Firefly
EC 1.13.12.7
Types de publication
Journal Article
Langues
eng
Sous-ensembles de citation
IM
Pagination
191-200Références
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