Methods of Lipid Analyses for Microalgae: Charophytes, Eustigmatophytes, and Euglenophytes.
Glycerolipids
Membrane lipids
Storage lipids
Surface lipids
Journal
Methods in molecular biology (Clifton, N.J.)
ISSN: 1940-6029
Titre abrégé: Methods Mol Biol
Pays: United States
ID NLM: 9214969
Informations de publication
Date de publication:
2021
2021
Historique:
entrez:
28
5
2021
pubmed:
29
5
2021
medline:
23
6
2021
Statut:
ppublish
Résumé
Algae are ecologically important organisms and are widely used for basic research, with a focus on for example photosynthesis, evolution, and lipid metabolism. Many biosynthetic pathways of algal lipids have been deciphered using available genomic information. Here we describe methods for lipid analyses from three representative algae, including Archaeplastida, the SAR lineage (Stramenopiles, Alveolata, Rhizaria), and Excavata. Archaeplastida acquired their plastids by primary endosymbiosis, and the others by secondary endosymbiosis with a Rhodophyceae-type plastid in SAR and a Chlorophyceae-type plastid in Excavata (Euglenozoa). Analytical methods for these algae are described for membrane lipids and neutral lipids including triacylglycerol and wax esters.
Identifiants
pubmed: 34047973
doi: 10.1007/978-1-0716-1362-7_6
doi:
Substances chimiques
Lipids
0
Types de publication
Journal Article
Research Support, Non-U.S. Gov't
Langues
eng
Sous-ensembles de citation
IM
Pagination
81-97Références
Li-Beisson Y, Thelen JJ, Fedosejevs E, Harwood JL (2019) The lipid biochemistry of eukaryotic algae. Prog Lipid Res 74:31–68. https://doi.org/10.1016/j.plipres.2019.01.003
doi: 10.1016/j.plipres.2019.01.003
pubmed: 30703388
Harwood JL (2019) Algae: critical sources of very long-chain polyunsaturated fatty acids. Biomol Ther 9(11):708. https://doi.org/10.3390/biom9110708
doi: 10.3390/biom9110708
Sakurai K, Mori N, Sato N (2014) Detection and characterization of phosphatidylcholine in various strains of the genus Chlamydomonas (Volvocales, Chlorophyceae). J Plant Res 127(5):641–650. https://doi.org/10.1007/s10265-014-0644-0
doi: 10.1007/s10265-014-0644-0
pubmed: 24947506
Canavate JP, Armada I, Rios JL, Hachero-Cruzado I (2016) Exploring occurrence and molecular diversity of betaine lipids across taxonomy of marine microalgae. Phytochemistry 124:68–78. https://doi.org/10.1016/j.phytochem.2016.02.007
doi: 10.1016/j.phytochem.2016.02.007
pubmed: 26895707
Kato M, Sakai M, Adachi K, Ikemoto H, Sano H (1996) Distribution of betaine lipids in marine algae. Phytochemistry 42(5):1341–1345. https://doi.org/10.1016/0031-9422(96)00115-X
doi: 10.1016/0031-9422(96)00115-X
Riekhof WR, Sears BB, Benning C (2005) Annotation of genes involved in glycerolipid biosynthesis in Chlamydomonas reinhardtii: discovery of the betaine lipid synthase BTA1Cr. Eukaryot Cell 4(2):242–252. https://doi.org/10.1128/EC.4.2.242-252.2005
doi: 10.1128/EC.4.2.242-252.2005
pubmed: 15701786
pmcid: 549322
Hori K, Nobusawa T, Watanabe T, Madoka Y, Suzuki H, Shibata D et al (2016) Tangled evolutionary processes with commonality and diversity in plastidial glycolipid synthesis in photosynthetic organisms. Biochim Biophys Acta 1861(9 Pt B):1294–1308. https://doi.org/10.1016/j.bbalip.2016.04.015
doi: 10.1016/j.bbalip.2016.04.015
pubmed: 27108062
Iwai M, Hori K, Sasaki-Sekimoto Y, Shimojima M, Ohta H (2015) Manipulation of oil synthesis in Nannochloropsis strain NIES-2145 with a phosphorus starvation-inducible promoter from Chlamydomonas reinhardtii. Front Microbiol 6:912. https://doi.org/10.3389/fmicb.2015.00912
doi: 10.3389/fmicb.2015.00912
pubmed: 26441858
pmcid: 4561341
Murakami H, Nobusawa T, Hori K, Shimojima M, Ohta H (2018) Betaine lipid is crucial for adapting to low temperature and phosphate deficiency in Nannochloropsis. Plant Physiol 177(1):181–193. https://doi.org/10.1104/pp.17.01573
doi: 10.1104/pp.17.01573
pubmed: 29555786
pmcid: 5933114
Shibata S, Arimura SI, Ishikawa T, Awai K (2018) Alterations of membrane lipid content correlated with chloroplast and mitochondria development in Euglena gracilis. Front Plant Sci 9:370. https://doi.org/10.3389/fpls.2018.00370
doi: 10.3389/fpls.2018.00370
pubmed: 29636759
pmcid: 5881160
Kondo S, Hori K, Sasaki-Sekimoto Y, Kobayashi A, Kato T, Yuno-Ohta N et al (2016) Primitive extracellular lipid components on the surface of the charophytic alga Klebsormidium flaccidum and their possible biosynthetic pathways as deduced from the genome sequence. Front Plant Sci 7:952. https://doi.org/10.3389/fpls.2016.00952
doi: 10.3389/fpls.2016.00952
pubmed: 27446179
pmcid: 4927632
Nishiyama T, Hiwatashi Y, Sakakibara I, Kato M, Hasebe M (2000) Tagged mutagenesis and gene-trap in the moss, Physcomitrella patens by shuttle mutagenesis. DNA Res 7(1):9–17. https://doi.org/10.1093/dnares/7.1.9
doi: 10.1093/dnares/7.1.9
pubmed: 10718194
Ichimura T (1971) Sexual cell division and conjugation-papilla formation in sexual reproduction of Closterium strigosum. University of Tokyo Press, Tokyo
Kilian O, Benemann CS, Niyogi KK, Vick B (2011) High-efficiency homologous recombination in the oil-producing alga Nannochloropsis sp. Proc Natl Acad Sci U S A 108(52):21265–21269. https://doi.org/10.1073/pnas.1105861108
doi: 10.1073/pnas.1105861108
pubmed: 22123974
pmcid: 3248512
Cramer M, Myers J (1952) Growth and photosynthetic characteristics of Euglena gracilis. Arch Mikrobiol 17(1):384–402. https://doi.org/10.1007/bf00410835
doi: 10.1007/bf00410835