The pluripotency transcription factor OCT4 represses heme oxygenase-1 gene expression.
Animals
Benzamides
/ pharmacology
Cell Differentiation
/ drug effects
Diphenylamine
/ analogs & derivatives
Embryo, Mammalian
Gene Expression Regulation
Green Fluorescent Proteins
/ genetics
Heme Oxygenase-1
/ genetics
Luciferases
/ genetics
Membrane Proteins
/ genetics
Mice
Mouse Embryonic Stem Cells
/ cytology
NIH 3T3 Cells
Nanog Homeobox Protein
/ antagonists & inhibitors
Octamer Transcription Factor-3
/ antagonists & inhibitors
Pluripotent Stem Cells
/ cytology
Promoter Regions, Genetic
Pyridines
/ pharmacology
Pyrimidines
/ pharmacology
RNA, Messenger
/ genetics
RNA, Small Interfering
/ genetics
SOXB1 Transcription Factors
/ antagonists & inhibitors
Signal Transduction
Transcription, Genetic
OCT4
gene modulation
heme oxygenase-1
pluripotency transcription factors
pluripotent stem cells
transcription
Journal
FEBS letters
ISSN: 1873-3468
Titre abrégé: FEBS Lett
Pays: England
ID NLM: 0155157
Informations de publication
Date de publication:
07 2021
07 2021
Historique:
revised:
17
05
2021
received:
14
04
2021
accepted:
23
05
2021
pubmed:
1
6
2021
medline:
5
8
2021
entrez:
31
5
2021
Statut:
ppublish
Résumé
In embryonic stem (ES) cells, oxidative stress control is crucial for genomic stability, self-renewal, and cell differentiation. Heme oxygenase-1 (HO-1) is a key player of the antioxidant system and is also involved in stem cell differentiation and pluripotency acquisition. We found that the HO-1 gene is expressed in ES cells and induced after promoting differentiation. Moreover, downregulation of the pluripotency transcription factor (TF) OCT4 increased HO-1 mRNA levels in ES cells, and analysis of ChIP-seq public data revealed that this TF binds to the HO-1 gene locus in pluripotent cells. Finally, ectopic expression of OCT4 in heterologous systems repressed a reporter carrying the HO-1 gene promoter and the endogenous gene. Hence, this work highlights the connection between pluripotency and redox homeostasis.
Identifiants
pubmed: 34056710
doi: 10.1002/1873-3468.14138
doi:
Substances chimiques
Benzamides
0
Chir 99021
0
Membrane Proteins
0
Nanog Homeobox Protein
0
Nanog protein, mouse
0
Octamer Transcription Factor-3
0
Pou5f1 protein, mouse
0
Pyridines
0
Pyrimidines
0
RNA, Messenger
0
RNA, Small Interfering
0
SOXB1 Transcription Factors
0
Sox2 protein, mouse
0
Green Fluorescent Proteins
147336-22-9
mirdametinib
86K0J5AK6M
Diphenylamine
9N3CBB0BIQ
Luciferases
EC 1.13.12.-
Heme Oxygenase-1
EC 1.14.14.18
Hmox1 protein, mouse
EC 1.14.14.18
Types de publication
Journal Article
Research Support, Non-U.S. Gov't
Langues
eng
Sous-ensembles de citation
IM
Pagination
1949-1961Informations de copyright
© 2021 Federation of European Biochemical Societies.
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