Assessing the activation/inhibition of tyrosine kinase-related pathways with a newly developed platform.
phosphorylation
protein array
signal transduction
Journal
Proteomics
ISSN: 1615-9861
Titre abrégé: Proteomics
Pays: Germany
ID NLM: 101092707
Informations de publication
Date de publication:
08 2021
08 2021
Historique:
revised:
16
06
2021
received:
30
09
2020
accepted:
18
06
2021
pubmed:
22
6
2021
medline:
26
10
2021
entrez:
21
6
2021
Statut:
ppublish
Résumé
The phosphorylation of cellular proteins plays a crucial role in the transduction of various signals from outside the cell into the nucleus. The signals are transduced by phosphorylation chain reactions within multiple pathways; however, determining which pathways are responsible for each defined signal has proven challenging. To estimate the activity of each pathway, we developed a phosphorylation array platform comprising a protein array with 1200 proteins belonging to 376 signalling pathways and an analytical method to estimate pathway activity based on the phosphorylation levels of proteins. The performance of our system was assessed by reconstructing kinase-substrate relationships, as well as by estimating pathway activity upon epidermal growth factor (EGF) stimulation and the pharmacological inhibition of epidermal growth factor receptor (EGFR). As a result, kinase-substrate relationships were reliably reconstructed based on the precise measurement of phosphorylation levels of constituent proteins on the array. Furthermore, the pathway activities associated with EGF stimulation and EGFR inhibition were successfully traced through the related pathways from the outer membrane to the nucleus along a time course. Thus, our phosphorylation array system can effectively assess the activity of specific signalling pathways that are perturbed by extracellular stimuli, such as various drugs.
Identifiants
pubmed: 34151541
doi: 10.1002/pmic.202000251
doi:
Substances chimiques
Tyrosine
42HK56048U
Epidermal Growth Factor
62229-50-9
Protein-Tyrosine Kinases
EC 2.7.10.1
Types de publication
Journal Article
Research Support, Non-U.S. Gov't
Langues
eng
Sous-ensembles de citation
IM
Pagination
e2000251Informations de copyright
© 2021 Wiley-VCH GmbH.
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