Androgen receptor variant shows heterogeneous expression in prostate cancer according to differentiation stage.
Journal
Communications biology
ISSN: 2399-3642
Titre abrégé: Commun Biol
Pays: England
ID NLM: 101719179
Informations de publication
Date de publication:
24 06 2021
24 06 2021
Historique:
received:
28
03
2020
accepted:
03
06
2021
entrez:
25
6
2021
pubmed:
26
6
2021
medline:
17
8
2021
Statut:
epublish
Résumé
Quantitation of androgen receptor variant (AR-V) expression in circulating tumor cells (CTCs) from patients with metastatic castration-resistant prostate cancer (mCRPC) has great potential for treatment customization. However, the absence of a uniform CTC isolation platform and consensus on an analytical assay has prevented the incorporation of these measurements in routine clinical practice. Here, we present a single-CTC sensitive digital droplet PCR (ddPCR) assay for the quantitation of the two most common AR-Vs, AR-V7, and AR-v567es, using antigen agnostic CTC enrichment. In a cohort of 29 mCRPC patients, we identify AR-V7 in 66% and AR-v567es in 52% of patients. These results are corroborated using another gene expression platform (NanoString
Identifiants
pubmed: 34168263
doi: 10.1038/s42003-021-02321-9
pii: 10.1038/s42003-021-02321-9
pmc: PMC8225618
doi:
Substances chimiques
AR protein, human
0
Receptors, Androgen
0
Types de publication
Journal Article
Research Support, N.I.H., Extramural
Research Support, U.S. Gov't, Non-P.H.S.
Langues
eng
Sous-ensembles de citation
IM
Pagination
785Subventions
Organisme : NCI NIH HHS
ID : R01 CA179100
Pays : United States
Organisme : NCI NIH HHS
ID : R21 CA216800
Pays : United States
Organisme : NCI NIH HHS
ID : T32 CA062948
Pays : United States
Organisme : NCI NIH HHS
ID : T32 CA203702
Pays : United States
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