Anther dehiscence is regulated by gibberellic acid in yellow lupine (Lupinus luteus L.).
Anther dehiscence
Degeneration via PCD
Endothecium
Gibberellins
Legumes
Secondary thickening
Septum/stomium rupture
Yellow lupine
Journal
BMC plant biology
ISSN: 1471-2229
Titre abrégé: BMC Plant Biol
Pays: England
ID NLM: 100967807
Informations de publication
Date de publication:
02 Jul 2021
02 Jul 2021
Historique:
received:
26
11
2020
accepted:
04
06
2021
entrez:
3
7
2021
pubmed:
4
7
2021
medline:
15
7
2021
Statut:
epublish
Résumé
Anther dehiscence resulting in the release of pollen grains is tightly regulated in a spatiotemporal manner by various factors. In yellow lupine (Lupinus luteus L.), a species that shows cleistogamy, the anthers split before the flowers open, but the course and regulation of this process are unknown. The specific control of anther development takes place via hormonal pathways, the wide action of which ensures reproductive success. In our previous research concerning flower and early pod development in yellow lupine, we showed that the lowest transcript level of LlDELLA1, a main repressor of gibberellin (GA) signalling, occurs approximately at the time of anther opening; therefore, the main purpose of this study was to precisely investigate the gibberellic acid (GA In this paper, we showed the specific changes in the yellow lupine anther structure during dehiscence, including secondary thickening in the endothecium by lignocellulosic deposition, enzymatic cell wall breakdown at the septum/stomium and cell degeneration via programmed cell death (PCD), and identified several genes widely associated with this process. The expression profile of genes varied over time, with the most intense mRNA accumulation in the phases prior to or at the time of anther opening. The transcriptional activity also revealed that these genes are highly coexpressed and regulated in a GA-dependent manner. The cellular and tissue localization of GA The presented results show a clear contribution of GA
Sections du résumé
BACKGROUND
BACKGROUND
Anther dehiscence resulting in the release of pollen grains is tightly regulated in a spatiotemporal manner by various factors. In yellow lupine (Lupinus luteus L.), a species that shows cleistogamy, the anthers split before the flowers open, but the course and regulation of this process are unknown. The specific control of anther development takes place via hormonal pathways, the wide action of which ensures reproductive success. In our previous research concerning flower and early pod development in yellow lupine, we showed that the lowest transcript level of LlDELLA1, a main repressor of gibberellin (GA) signalling, occurs approximately at the time of anther opening; therefore, the main purpose of this study was to precisely investigate the gibberellic acid (GA
RESULTS
RESULTS
In this paper, we showed the specific changes in the yellow lupine anther structure during dehiscence, including secondary thickening in the endothecium by lignocellulosic deposition, enzymatic cell wall breakdown at the septum/stomium and cell degeneration via programmed cell death (PCD), and identified several genes widely associated with this process. The expression profile of genes varied over time, with the most intense mRNA accumulation in the phases prior to or at the time of anther opening. The transcriptional activity also revealed that these genes are highly coexpressed and regulated in a GA-dependent manner. The cellular and tissue localization of GA
CONCLUSIONS
CONCLUSIONS
The presented results show a clear contribution of GA
Identifiants
pubmed: 34215194
doi: 10.1186/s12870-021-03085-4
pii: 10.1186/s12870-021-03085-4
pmc: PMC8252261
doi:
Substances chimiques
Gibberellins
0
Plant Proteins
0
gibberellic acid
BU0A7MWB6L
Types de publication
Journal Article
Langues
eng
Sous-ensembles de citation
IM
Pagination
314Subventions
Organisme : Polish Ministry of Agriculture and Rural Development
ID : 149/2011 and 222/2015
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