Dissection of a rice OsMac1 mRNA 5' UTR to uncover regulatory elements that are responsible for its efficient translation.
Journal
PloS one
ISSN: 1932-6203
Titre abrégé: PLoS One
Pays: United States
ID NLM: 101285081
Informations de publication
Date de publication:
2021
2021
Historique:
received:
19
03
2021
accepted:
07
06
2021
entrez:
9
7
2021
pubmed:
10
7
2021
medline:
29
10
2021
Statut:
epublish
Résumé
The untranslated regions (UTRs) of mRNAs are involved in many posttranscriptional regulatory pathways. The rice OsMac1 mRNA has three splicing variants of the 5' UTR (UTRa, UTRb, and UTRc), which include a CU-rich region and three upstream open reading frames (uORFs). UTRc contains an additional 38-nt sequence, termed sp38, which acts as a strong translational enhancer of the downstream ORF; reporter analysis revealed translational efficiencies >15-fold higher with UTRc than with the other splice variants. Mutation analysis of UTRc demonstrated that an optimal sequence length of sp38, rather than its nucleotide sequence is essential for UTRc to promote efficient translation. In addition, the 5' 100 nucleotides of CU-rich region contribute to UTRc translational enhancement. Strikingly, three uORFs did not reveal their inhibitory potential within the full-length leader, whereas deletion of the 5' leader fragment preceding the leader region with uORFs nearly abolished translation. Computational prediction of UTRc structural motifs revealed stem-loop structures, termed SL1-SL4, and two regions, A and B, involved in putative intramolecular interactions. Our data suggest that SL4 binding to Region-A and base pairing between Region-B and the UTRc 3'end are critically required for translational enhancement. Since UTRc is not capable of internal initiation, we presume that the three-dimensional leader structures can allow translation of the leader downstream ORF, likely allowing the bypass of uORFs.
Identifiants
pubmed: 34242244
doi: 10.1371/journal.pone.0253488
pii: PONE-D-21-09082
pmc: PMC8270207
doi:
Substances chimiques
5' Untranslated Regions
0
RNA, Messenger
0
Types de publication
Journal Article
Research Support, Non-U.S. Gov't
Langues
eng
Sous-ensembles de citation
IM
Pagination
e0253488Déclaration de conflit d'intérêts
No authors have competing interests.
Références
Nat Rev Mol Cell Biol. 2004 Oct;5(10):827-35
pubmed: 15459663
Plant Physiol. 2009 Jul;150(3):1356-67
pubmed: 19403731
Virology. 1999 Oct 10;263(1):139-54
pubmed: 10544089
J Cell Biochem. 2007 Oct 1;102(2):280-90
pubmed: 17647274
Proc Natl Acad Sci U S A. 2009 May 5;106(18):7507-12
pubmed: 19372376
Nat Protoc. 2007;2(7):1565-72
pubmed: 17585298
Plant Cell Physiol. 2009 Feb;50(2):179-90
pubmed: 19122186
Plant Cell. 2010 Oct;22(10):3280-94
pubmed: 20889913
Nucleic Acids Res. 2010 Nov;38(20):7068-78
pubmed: 20576698
Gene. 1998 Oct 9;221(1):35-43
pubmed: 9852947
Nucleic Acids Res. 2000 Aug 1;28(15):2927-34
pubmed: 10908356
Mol Cell Biol. 2008 Mar;28(6):2011-22
pubmed: 18195037
J Biosci Bioeng. 2007 Jul;104(1):34-41
pubmed: 17697981
Genomics. 2000 Dec 15;70(3):396-406
pubmed: 11161792
J Biol Chem. 2000 Jun 9;275(23):17288-96
pubmed: 10747993
Ann N Y Acad Sci. 2004 Dec;1038:60-74
pubmed: 15838098
Mol Cell. 2008 May 23;30(4):460-71
pubmed: 18498749
Biol Cell. 2009 May;101(5):251-62
pubmed: 19275763
Genome Biol. 2002;3(3):REVIEWS0004
pubmed: 11897027
Anal Biochem. 1997 Dec 15;254(2):157-78
pubmed: 9417773
Gene. 2001 Oct 3;276(1-2):73-81
pubmed: 11591473
Plant Cell Physiol. 2007 Aug;48(8):1108-20
pubmed: 17609219
Plant Cell Environ. 2015 Oct;38(10):2098-114
pubmed: 25764476
Front Microbiol. 2018 Apr 10;9:644
pubmed: 29692761
Cell. 1986 Jan 31;44(2):283-92
pubmed: 3943125
EMBO J. 2005 Nov 2;24(21):3737-46
pubmed: 16222335
Gene. 1999 Jul 8;234(2):187-208
pubmed: 10395892
Life Sci. 2018 Nov 1;212:138-144
pubmed: 30290184
Plant J. 2005 Mar;41(5):722-31
pubmed: 15703059
EMBO J. 1987 Dec 20;6(13):3901-7
pubmed: 3327686
J Biol Chem. 2005 Nov 25;280(47):39229-37
pubmed: 16176926
Nature. 2015 Jan 1;517(7532):33-8
pubmed: 25409156
Plant Physiol. 2018 Feb;176(2):1095-1105
pubmed: 29122989
Mol Cell Biol. 2008 Jan;28(1):93-107
pubmed: 17967866
Biotechnol Genet Eng Rev. 2018 Apr;34(1):60-75
pubmed: 29804514
BMC Genomics. 2008 Jul 31;9:361
pubmed: 18667093
J Biol Chem. 2005 Jul 22;280(29):26813-24
pubmed: 15911616
Nat Rev Mol Cell Biol. 2010 Feb;11(2):113-27
pubmed: 20094052