Aptamer-based assays: strategies in the use of aptamers conjugated to magnetic micro- and nanobeads as recognition elements in food control.
Aptamer
Electrochemical aptasensor
Food control
Magnetic beads
Magnetic solid-phase extraction
Optical apta-assay
Journal
Analytical and bioanalytical chemistry
ISSN: 1618-2650
Titre abrégé: Anal Bioanal Chem
Pays: Germany
ID NLM: 101134327
Informations de publication
Date de publication:
Jan 2022
Jan 2022
Historique:
received:
10
05
2021
accepted:
23
06
2021
revised:
18
06
2021
pubmed:
11
7
2021
medline:
18
3
2022
entrez:
10
7
2021
Statut:
ppublish
Résumé
An outlook on the current status of different strategies for magnetic micro- and nanosized bead functionalization with aptamers as prominent bioreceptors is given with a focus on electrochemical and optical apta-assays, as well as on aptamer-modified magnetic bead-based miniaturized extraction techniques in food control. Critical aspects that affect interaction of aptamers with target molecules, as well as the possible side effects caused by aptamer interaction with other molecules due to non-specific binding, are discussed. Challenges concerning the real potential and limitations of aptamers as bioreceptors when facing analytical problems in food control are addressed.
Identifiants
pubmed: 34245326
doi: 10.1007/s00216-021-03501-6
pii: 10.1007/s00216-021-03501-6
pmc: PMC8748373
doi:
Substances chimiques
Aptamers, Nucleotide
0
Gold
7440-57-5
Types de publication
Journal Article
Langues
eng
Sous-ensembles de citation
IM
Pagination
63-74Subventions
Organisme : Ministero dell'Istruzione, dell'Università e della Ricerca
ID : PRIN 2017 (Progetti di Ricerca di Rilevante Intere
Informations de copyright
© 2021. The Author(s).
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