CCTδ colocalizes with actin and β-tubulin: Insight into its involvement in the cytoskeleton formation of the intracellular parasite Nosema bombycis.

Actins / genetics Chaperonin Containing TCP-1 / genetics Cytoskeleton / physiology Fungal Proteins / genetics Nosema / genetics Tubulin / genetics
CCT Chaperonin Immunolocalization Nosema bombycis RNAi

Journal

Journal of invertebrate pathology
ISSN: 1096-0805
Titre abrégé: J Invertebr Pathol
Pays: United States
ID NLM: 0014067

Informations de publication

Date de publication:
09 2021
Historique:
received: 24 04 2021
revised: 27 06 2021
accepted: 05 07 2021
pubmed: 14 7 2021
medline: 30 11 2021
entrez: 13 7 2021
Statut: ppublish

Résumé

The chaperonin-containing t-complex polypeptide 1 (CCT) is a molecular chaperone protein that is widely present in eukaryotic cytoplasm and can assist in the folding of newly synthesized proteins. The CCT complex consists of eight completely different subunits, among which the δ subunit plays an extremely important role in the folding and assembly of cytoskeleton proteins as an individual or complex with other subunits. In this study, we identified the CCTδ in the microsporidian Nosema bombycis (NbCCTδ) for the first time. The NbCCTδ gene contains a complete ORF of 1497 bp in length that encodes a 498 amino acid polypeptide. NbCCTδ is expressed throughout the entire lifecycle of N. bombycis and rather higher in early stage of proliferation. Indirect immunofluorescence results showed that NbCCTδ was colocalized with actin and β-tubulin during the proliferative and sporogonic phases of N. bombycis. RNA interference down-regulated the expression of the NbCCTδ gene. These results imply that NbCCTδ may participate in cytoskeleton formation and proliferation of N. bombycis.

Identifiants

DOI: 10.1016/j.jip.2021.107646 PMID: 34256048
pubmed: 34256048
pii: S0022-2011(21)00113-0
doi: 10.1016/j.jip.2021.107646
pii:
doi:

Substances chimiques

Actins 0
Fungal Proteins 0
Tubulin 0
Chaperonin Containing TCP-1 EC 3.6.1.-

Types de publication

Journal Article Research Support, Non-U.S. Gov't

Langues

eng

Sous-ensembles de citation

IM

Pagination

107646

Informations de copyright

Copyright © 2021 Elsevier Inc. All rights reserved.

Auteurs

Jingru Qi (J)

Jiangsu University of Science and Technology, Zhenjiang 212018, Jiangsu Province, China.

Feng Zhu (F)

College of Life Sciences, Zaozhuang University, Zaozhuang 277160, Shandong Province, China; Institute of Sericulture and Apiculture, Yunnan Academy of Agricultural Sciences, Mengzi 661101, Yunnan Province, China.

Li Shao (L)

College of Life Sciences, Zaozhuang University, Zaozhuang 277160, Shandong Province, China.

Ying Chen (Y)

Jiangsu University of Science and Technology, Zhenjiang 212018, Jiangsu Province, China.

Jicheng Li (J)

College of Life Sciences, Zaozhuang University, Zaozhuang 277160, Shandong Province, China.

Ping He (P)

Jiangsu University of Science and Technology, Zhenjiang 212018, Jiangsu Province, China.

Ruisha Shang (R)

Jiangsu University of Science and Technology, Zhenjiang 212018, Jiangsu Province, China.

Fuzhen Sun (F)

Jiangsu University of Science and Technology, Zhenjiang 212018, Jiangsu Province, China.

Qiang Wang (Q)

Jiangsu University of Science and Technology, Zhenjiang 212018, Jiangsu Province, China; Sericultural Research Institute, Chinese Academy of Agricultural Sciences, Zhenjiang 212018, Jiangsu Province, China.

Yiling Zhang (Y)

Jiangsu University of Science and Technology, Zhenjiang 212018, Jiangsu Province, China; Sericultural Research Institute, Chinese Academy of Agricultural Sciences, Zhenjiang 212018, Jiangsu Province, China.

Xudong Tang (X)

Jiangsu University of Science and Technology, Zhenjiang 212018, Jiangsu Province, China; Sericultural Research Institute, Chinese Academy of Agricultural Sciences, Zhenjiang 212018, Jiangsu Province, China.

Zhongyuan Shen (Z)

Jiangsu University of Science and Technology, Zhenjiang 212018, Jiangsu Province, China; Sericultural Research Institute, Chinese Academy of Agricultural Sciences, Zhenjiang 212018, Jiangsu Province, China. Electronic address: szysri@163.com.

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Classifications MeSH

questionsmedicales.fr › Acides aminés, peptides et protéines › Protéines › Protéines du cytosquelette › Protéines des microfilaments › Actines › CCTδ colocalizes with actin and β-tubulin:...
questionsmedicales.fr › Acides aminés, peptides et protéines › Protéines › Chaperons moléculaires › Protéines du choc thermique › Chaperonines › Chaperonines du groupe II › Chaperonine contenant TCP-1 › CCTδ colocalizes with actin and β-tubulin:...
questionsmedicales.fr › Cellules › Structures cellulaires › Espace intracellulaire › Cytoplasme › Structures cytoplasmiques › Cytosquelette › CCTδ colocalizes with actin and β-tubulin:...
questionsmedicales.fr › Acides aminés, peptides et protéines › Protéines › Protéines fongiques › CCTδ colocalizes with actin and β-tubulin:...
questionsmedicales.fr › Eucaryotes › Champignons › Microsporidia › Microsporea › Microsporida › Apansporoblastina › Nosema › CCTδ colocalizes with actin and β-tubulin:...
questionsmedicales.fr › Acides aminés, peptides et protéines › Protéines › Protéines de tissu nerveux › Tubuline › CCTδ colocalizes with actin and β-tubulin:...