Two-Photon, Ratiometric, Quantitative Fluorescent Probe Reveals Fluctuation of Peroxynitrite Regulated by Arginase 1.


Journal

Analytical chemistry
ISSN: 1520-6882
Titre abrégé: Anal Chem
Pays: United States
ID NLM: 0370536

Informations de publication

Date de publication:
27 07 2021
Historique:
pubmed: 17 7 2021
medline: 10 8 2021
entrez: 16 7 2021
Statut: ppublish

Résumé

Peroxynitrite, a transient reactive oxygen species (ROS), is believed to play a deleterious role in physiological processes. Herein, we report a two-photon ratiometric fluorescent probe that selectively reacts with peroxynitrite yielding a >200-fold change upon reaction. The probe effectively visualized fluctuations in peroxynitrite generation by arginase 1 in vivo and in vitro. This provides evidence that arginase 1 is a critical regulator of peroxynitrite.

Identifiants

pubmed: 34269045
doi: 10.1021/acs.analchem.1c00911
doi:

Substances chimiques

Fluorescent Dyes 0
Reactive Oxygen Species 0
Peroxynitrous Acid 14691-52-2
Arginase EC 3.5.3.1

Types de publication

Journal Article Research Support, N.I.H., Extramural Research Support, U.S. Gov't, Non-P.H.S.

Langues

eng

Sous-ensembles de citation

IM

Pagination

10090-10098

Auteurs

Shiyu Chen (S)

Department of Chemistry, New York University, New York, New York 10003, United States.

Beyza Vurusaner (B)

Department of Medicine (Cardiology) and Cell Biology, Marc and Ruti Bell Program in Vascular Biology, NYU School of Medicine, New York, New York 10016, United States.

Stephanie Pena (S)

Department of Medicine (Cardiology) and Cell Biology, Marc and Ruti Bell Program in Vascular Biology, NYU School of Medicine, New York, New York 10016, United States.

Chu T Thu (CT)

Department of Chemistry, New York University, New York, New York 10003, United States.

Lara K Mahal (LK)

Department of Chemistry, New York University, New York, New York 10003, United States.

Edward A Fisher (EA)

Department of Medicine (Cardiology) and Cell Biology, Marc and Ruti Bell Program in Vascular Biology, NYU School of Medicine, New York, New York 10016, United States.

James W Canary (JW)

Department of Chemistry, New York University, New York, New York 10003, United States.

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Classifications MeSH