Deciphering Mesenchymal Drivers of Human Dupuytren's Disease at Single-Cell Level.
Basic Helix-Loop-Helix Transcription Factors
/ genetics
Cell Differentiation
Cell Proliferation
Cells, Cultured
Dermis
/ physiology
Dupuytren Contracture
/ genetics
Endopeptidases
/ metabolism
Fibroblasts
/ physiology
Fibrosis
/ genetics
Gene Expression Profiling
Gene Knockdown Techniques
Humans
Membrane Glycoproteins
/ metabolism
Membrane Proteins
/ metabolism
Mesenchymal Stem Cells
/ physiology
Myofibrils
/ pathology
Sequence Analysis, RNA
Single-Cell Analysis
TWEAK Receptor
/ genetics
Journal
The Journal of investigative dermatology
ISSN: 1523-1747
Titre abrégé: J Invest Dermatol
Pays: United States
ID NLM: 0426720
Informations de publication
Date de publication:
01 2022
01 2022
Historique:
received:
01
03
2021
revised:
24
05
2021
accepted:
25
05
2021
pubmed:
19
7
2021
medline:
15
1
2022
entrez:
18
7
2021
Statut:
ppublish
Résumé
Dupuytren's disease (DD) is a common, progressive fibroproliferative disease affecting the palmar fascia of the hands, causing fingers to irreversibly flex toward the palm with significant loss of function. Surgical treatments are limited; therefore, effective new therapies for DD are urgently required. To identify the key cellular and molecular pathways driving DD, we employed single-cell RNA sequencing, profiling the transcriptomes of 35,250 human single cells from DD, nonpathogenic fascia, and healthy dermis. We identify a DD-specific population of pathogenic PDPN
Identifiants
pubmed: 34274346
pii: S0022-202X(21)01453-6
doi: 10.1016/j.jid.2021.05.030
pii:
doi:
Substances chimiques
Basic Helix-Loop-Helix Transcription Factors
0
Membrane Glycoproteins
0
Membrane Proteins
0
PDPN protein, human
0
SCX protein, human
0
TNFRSF12A protein, human
0
TWEAK Receptor
0
Endopeptidases
EC 3.4.-
fibroblast activation protein alpha
EC 3.4.21.-
Types de publication
Journal Article
Research Support, Non-U.S. Gov't
Langues
eng
Sous-ensembles de citation
IM
Pagination
114-123.e8Subventions
Organisme : Cancer Research UK
ID : 24867
Pays : United Kingdom
Organisme : Medical Research Council
ID : MR/W015919/1
Pays : United Kingdom
Organisme : Wellcome Trust
ID : 219542/Z/19/Z
Pays : United Kingdom
Organisme : Medical Research Council
ID : MR/N008340/1
Pays : United Kingdom
Informations de copyright
Copyright © 2021 The Authors. Published by Elsevier Inc. All rights reserved.