A search for modifying genetic factors in CHEK2:c.1100delC breast cancer patients.


Journal

Scientific reports
ISSN: 2045-2322
Titre abrégé: Sci Rep
Pays: England
ID NLM: 101563288

Informations de publication

Date de publication:
20 07 2021
Historique:
received: 18 04 2021
accepted: 28 06 2021
entrez: 21 7 2021
pubmed: 22 7 2021
medline: 16 11 2021
Statut: epublish

Résumé

The risk of breast cancer associated with CHEK2:c.1100delC is 2-threefold but higher in carriers with a family history of breast cancer than without, suggesting that other genetic loci in combination with CHEK2:c.1100delC confer an increased risk in a polygenic model. Part of the excess familial risk has been associated with common low-penetrance variants. This study aimed to identify genetic loci that modify CHEK2:c.1100delC-associated breast cancer risk by searching for candidate risk alleles that are overrepresented in CHEK2:c.1100delC carriers with breast cancer compared with controls. We performed whole-exome sequencing in 28 breast cancer cases with germline CHEK2:c.1100delC, 28 familial breast cancer cases and 70 controls. Candidate alleles were selected for validation in larger cohorts. One recessive synonymous variant, rs16897117, was suggested, but no overrepresentation of homozygous CHEK2:c.1100delC carriers was found in the following validation. Furthermore, 11 non-synonymous candidate alleles were suggested for further testing, but no significant difference in allele frequency could be detected in the validation in CHEK2:c.1100delC cases compared with familial breast cancer, sporadic breast cancer and controls. With this method, we found no support for a CHEK2:c.1100delC-specific genetic modifier. Further studies of CHEK2:c.1100delC genetic modifiers are warranted to improve risk assessment in clinical practice.

Identifiants

pubmed: 34285278
doi: 10.1038/s41598-021-93926-x
pii: 10.1038/s41598-021-93926-x
pmc: PMC8292481
doi:

Substances chimiques

Checkpoint Kinase 2 EC 2.7.1.11
CHEK2 protein, human EC 2.7.11.1

Types de publication

Journal Article Research Support, Non-U.S. Gov't

Langues

eng

Sous-ensembles de citation

IM

Pagination

14763

Informations de copyright

© 2021. The Author(s).

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Auteurs

Camilla Wendt (C)

Department of Clinical Science and Education, Karolinska Institutet, Södersjukhuset, Stockholm, Sweden. camilla.wendt@sll.se.

Taru A Muranen (TA)

Department of Obstetrics and Gynecology, Helsinki University Hospital, University of Helsinki, Helsinki, Finland.

Lotta Mielikäinen (L)

Department of Obstetrics and Gynecology, Helsinki University Hospital, University of Helsinki, Helsinki, Finland.

Jessada Thutkawkorapin (J)

Department of Molecular Medicine and Surgery, Karolinska Institutet, Solna, Stockholm, Sweden.

Carl Blomqvist (C)

Department of Oncology, Helsinki University Hospital, University of Helsinki, Helsinki, Finland.

Xiang Jiao (X)

Department of Molecular Medicine and Surgery, Karolinska Institutet, Solna, Stockholm, Sweden.

Hans Ehrencrona (H)

Department of Clinical Genetics and Pathology, Office for Medical Services, Region Skåne, Lund, Sweden.

Emma Tham (E)

Department of Molecular Medicine and Surgery, Karolinska Institutet, Solna, Stockholm, Sweden.

Brita Arver (B)

Department of Oncology-Pathology, Karolinska Institutet, Solna, Stockholm, Sweden.

Beatrice Melin (B)

Department of Radiation Sciences, Oncology, Umeå University, Umeå, Sweden.

Ekaterina Kuchinskaya (E)

Department of Clinical Genetics, Department of Clinical Experimental Medicine, Linköping University, Linköping, Sweden.

Marie Stenmark Askmalm (M)

Department of Clinical Genetics, Department of Clinical Experimental Medicine, Linköping University, Linköping, Sweden.

Ylva Paulsson-Karlsson (Y)

Department of Immunology, Genetics and Pathology, Uppsala University, Uppsala, Sweden.

Zakaria Einbeigi (Z)

Department of Oncology, Sahlgrenska University Hospital, 41345, Göteborg, Sweden.

Anna von Wachenfeldt Väppling (A)

Department of Clinical Science and Education, Karolinska Institutet, Södersjukhuset, Stockholm, Sweden.

Eija Kalso (E)

Department of Anaesthesiology, Intensive Care, and Pain Medicine, Helsinki University Hospital and University of Helsinki, Helsinki, Finland.

Tiina Tasmuth (T)

Department of Anaesthesiology, Intensive Care, and Pain Medicine, Helsinki University Hospital and University of Helsinki, Helsinki, Finland.

Anne Kallioniemi (A)

TAYS Cancer Centre and Faculty of Medicine and Health Technology, Tampere University; Fimlab Laboratories, Tampere University Hospital, Tampere, Finland.

Kristiina Aittomäki (K)

Department of Medical and Clinical Genetics, University of Helsinki, Helsinki, Finland.

Heli Nevanlinna (H)

Department of Obstetrics and Gynecology, Helsinki University Hospital, University of Helsinki, Helsinki, Finland.

Åke Borg (Å)

Department of Oncology and Pathology, Department of Clinical Sciences Lund, Lund University, Medicon Village, Lund, Sweden.

Annika Lindblom (A)

Department of Molecular Medicine and Surgery, Karolinska Institutet, Solna, Stockholm, Sweden.

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Classifications MeSH