Rational construction of genome-reduced Burkholderiales chassis facilitates efficient heterologous production of natural products from proteobacteria.
Biological Products
/ metabolism
Burkholderiaceae
/ genetics
Burkholderiales
/ genetics
Escherichia coli
/ genetics
Gene Expression Regulation, Bacterial
Genetic Engineering
/ methods
Genome, Bacterial
/ genetics
Multigene Family
/ genetics
Mutation
Polyketides
/ metabolism
Proteobacteria
/ genetics
Pseudomonas putida
/ genetics
Journal
Nature communications
ISSN: 2041-1723
Titre abrégé: Nat Commun
Pays: England
ID NLM: 101528555
Informations de publication
Date de publication:
23 07 2021
23 07 2021
Historique:
received:
02
11
2020
accepted:
29
06
2021
entrez:
24
7
2021
pubmed:
25
7
2021
medline:
11
8
2021
Statut:
epublish
Résumé
Heterologous expression of biosynthetic gene clusters (BGCs) avails yield improvements and mining of natural products, but it is limited by lacking of more efficient Gram-negative chassis. The proteobacterium Schlegelella brevitalea DSM 7029 exhibits potential for heterologous BGC expression, but its cells undergo early autolysis, hindering further applications. Herein, we rationally construct DC and DT series genome-reduced S. brevitalea mutants by sequential deletions of endogenous BGCs and the nonessential genomic regions, respectively. The DC5 to DC7 mutants affect growth, while the DT series mutants show improved growth characteristics with alleviated cell autolysis. The yield improvements of six proteobacterial natural products and successful identification of chitinimides from Chitinimonas koreensis via heterologous expression in DT mutants demonstrate their superiority to wild-type DSM 7029 and two commonly used Gram-negative chassis Escherichia coli and Pseudomonas putida. Our study expands the panel of Gram-negative chassis and facilitates the discovery of natural products by heterologous expression.
Identifiants
pubmed: 34301933
doi: 10.1038/s41467-021-24645-0
pii: 10.1038/s41467-021-24645-0
pmc: PMC8302735
doi:
Substances chimiques
Biological Products
0
Polyketides
0
Types de publication
Journal Article
Research Support, Non-U.S. Gov't
Langues
eng
Sous-ensembles de citation
IM
Pagination
4347Informations de copyright
© 2021. The Author(s).
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