Biodegradation of metoprolol in oxic and anoxic hyporheic zone sediments: unexpected effects on microbial communities.
16S rRNA microbiome analysis
Hyporheic zone
Metoprolol
Micropollutant transformation
Journal
Applied microbiology and biotechnology
ISSN: 1432-0614
Titre abrégé: Appl Microbiol Biotechnol
Pays: Germany
ID NLM: 8406612
Informations de publication
Date de publication:
Aug 2021
Aug 2021
Historique:
received:
18
02
2021
accepted:
20
07
2021
revised:
19
07
2021
pubmed:
3
8
2021
medline:
1
9
2021
entrez:
2
8
2021
Statut:
ppublish
Résumé
Metoprolol is widely used as a beta-blocker and considered an emerging contaminant of environmental concern due to pseudo persistence in wastewater effluents that poses a potential ecotoxicological threat to aquatic ecosystems. Microbial removal of metoprolol in the redox-delineated hyporheic zone (HZ) was investigated using streambed sediments supplemented with 15 or 150 μM metoprolol in a laboratory microcosm incubation under oxic and anoxic conditions. Metoprolol disappeared from the aqueous phase under oxic and anoxic conditions within 65 and 72 days, respectively. Metoprolol was refed twice after initial depletion resulting in accelerated disappearance under both conditions. Metoprolol disappearance was marginal in sterile control microcosms with autoclaved sediment. Metoprolol was transformed mainly to metoprolol acid in oxic microcosms, while metoprolol acid and α-hydroxymetoprolol were formed in anoxic microcosms. Transformation products were transient and disappeared within 30 days under both conditions. Effects of metoprolol on the HZ bacterial community were evaluated using DNA- and RNA-based time-resolved amplicon Illumina MiSeq sequencing targeting the 16S rRNA gene and 16S rRNA, respectively, and were prominent on 16S rRNA rather than 16S rRNA gene level suggesting moderate metoprolol-induced activity-level changes. A positive impact of metoprolol on Sphingomonadaceae and Enterobacteriaceae under oxic and anoxic conditions, respectively, was observed. Nitrifiers were impaired by metoprolol under oxic and anoxic conditions. Collectively, our findings revealed high metoprolol biodegradation potentials in the hyporheic zone under contrasting redox conditions associated with changes in the active microbial communities, thus contributing to the attenuation of micropollutants. KEY POINTS: • High biotic oxic and anoxic metoprolol degradation potentials in the hyporheic zone. • Key metoprolol-associated taxa included Sphingomonadaceae, Enterobacteraceae, and Promicromonosporaceae. • Negative impact of metoprolol on nitrifiers.
Identifiants
pubmed: 34338804
doi: 10.1007/s00253-021-11466-w
pii: 10.1007/s00253-021-11466-w
pmc: PMC8390428
doi:
Substances chimiques
RNA, Ribosomal, 16S
0
Metoprolol
GEB06NHM23
Types de publication
Journal Article
Langues
eng
Sous-ensembles de citation
IM
Pagination
6103-6115Subventions
Organisme : H2020 European Research Council
ID : 641939
Informations de copyright
© 2021. The Author(s).
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