Abnormality in the NK-cell population is prolonged in severe COVID-19 patients.
COVID-19
NK cells
SARS-CoV-2
cytotoxicity
innate immunity
unconventional CD56(dim)CD16(neg) (uCD56(dim)) NK cell
Journal
The Journal of allergy and clinical immunology
ISSN: 1097-6825
Titre abrégé: J Allergy Clin Immunol
Pays: United States
ID NLM: 1275002
Informations de publication
Date de publication:
10 2021
10 2021
Historique:
received:
21
02
2021
revised:
27
06
2021
accepted:
21
07
2021
pubmed:
3
8
2021
medline:
21
10
2021
entrez:
2
8
2021
Statut:
ppublish
Résumé
Our understanding of adaptive immune responses in patients with coronavirus disease 2019 (COVID-19) is rapidly evolving, but information on the innate immune responses by natural killer (NK) cells is still insufficient. We aimed to examine the phenotypic and functional status of NK cells and their changes during the course of mild and severe COVID-19. We performed RNA sequencing and flow cytometric analysis of NK cells from patients with mild and severe COVID-19 at multiple time points in the course of the disease using cryopreserved PBMCs. In RNA-sequencing analysis, the NK cells exhibited distinctive features compared with healthy donors, with significant enrichment of proinflammatory cytokine-mediated signaling pathways. Intriguingly, we found that the unconventional CD56 The current longitudinal study provides a deep understanding of the NK-cell biology in COVID-19.
Sections du résumé
BACKGROUND
Our understanding of adaptive immune responses in patients with coronavirus disease 2019 (COVID-19) is rapidly evolving, but information on the innate immune responses by natural killer (NK) cells is still insufficient.
OBJECTIVE
We aimed to examine the phenotypic and functional status of NK cells and their changes during the course of mild and severe COVID-19.
METHODS
We performed RNA sequencing and flow cytometric analysis of NK cells from patients with mild and severe COVID-19 at multiple time points in the course of the disease using cryopreserved PBMCs.
RESULTS
In RNA-sequencing analysis, the NK cells exhibited distinctive features compared with healthy donors, with significant enrichment of proinflammatory cytokine-mediated signaling pathways. Intriguingly, we found that the unconventional CD56
CONCLUSIONS
The current longitudinal study provides a deep understanding of the NK-cell biology in COVID-19.
Identifiants
pubmed: 34339730
pii: S0091-6749(21)01142-8
doi: 10.1016/j.jaci.2021.07.022
pmc: PMC8324384
pii:
doi:
Types de publication
Journal Article
Langues
eng
Sous-ensembles de citation
IM
Pagination
996-1006.e18Informations de copyright
Copyright © 2021 American Academy of Allergy, Asthma & Immunology. Published by Elsevier Inc. All rights reserved.
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