GP.MOT: A novel glycophorin variant identified in a Japanese blood donor.
MNS blood groups
glycophorin A (GPA)
glycophorin B (GPB)
glycophorin variant
Journal
Transfusion
ISSN: 1537-2995
Titre abrégé: Transfusion
Pays: United States
ID NLM: 0417360
Informations de publication
Date de publication:
10 2021
10 2021
Historique:
revised:
29
03
2021
received:
14
12
2020
accepted:
05
04
2021
pubmed:
10
8
2021
medline:
5
11
2021
entrez:
9
8
2021
Statut:
ppublish
Résumé
In this study, we identified a novel glycophorin variant (GP.MOT) in a Mi Standard serological techniques and flow cytometry were performed. GP.MOT RBCs were examined by immunoblotting using anti-GPA, anti-MUT or anti-Mur. Genome DNA was extracted from whole blood, and the GYPA/GYPB was analyzed by polymerase chain reactions and Sanger sequencing. The MNS blood group of the proband was M + N + We identified a hybrid glycophorin GP.MOT with the deduced unique amino acid sequence GPB (20-45)-GPΨB (46-70)-GPA (71-149), which has not been previously reported.
Sections du résumé
BACKGROUND
In this study, we identified a novel glycophorin variant (GP.MOT) in a Mi
STUDY DESIGN AND METHODS
Standard serological techniques and flow cytometry were performed. GP.MOT RBCs were examined by immunoblotting using anti-GPA, anti-MUT or anti-Mur. Genome DNA was extracted from whole blood, and the GYPA/GYPB was analyzed by polymerase chain reactions and Sanger sequencing.
RESULTS
The MNS blood group of the proband was M + N +
DISCUSSION
We identified a hybrid glycophorin GP.MOT with the deduced unique amino acid sequence GPB (20-45)-GPΨB (46-70)-GPA (71-149), which has not been previously reported.
Substances chimiques
GYPA protein, human
0
GYPB protein, human
0
Glycophorins
0
MNSs Blood-Group System
0
Types de publication
Case Reports
Journal Article
Langues
eng
Sous-ensembles de citation
IM
Pagination
2825-2829Informations de copyright
© 2021 AABB.
Références
The International Society of Blood Transfusion: Red Cell Immunogenetics and Blood Terminology Working Party. https://www.isbtweb.org/filiadmin/user_upload/table_of_blood_group_antigens_with_win_systems_9.0-203-FEdps_203-2021
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