Chemical Protein Synthesis by Chemoselective #x03B1;-Ketoacid-Hydroxylamine (KAHA) Ligations with 5-Oxaproline.
5-Oxaproline
Hydroxylamine
KAHA ligation
SPPS
Ubiquitin
α-Ketoacid
Journal
Methods in molecular biology (Clifton, N.J.)
ISSN: 1940-6029
Titre abrégé: Methods Mol Biol
Pays: United States
ID NLM: 9214969
Informations de publication
Date de publication:
2021
2021
Historique:
entrez:
13
8
2021
pubmed:
14
8
2021
medline:
12
1
2022
Statut:
ppublish
Résumé
Chemical protein synthesis enables the precise construction of proteins by employing solid-phase peptide synthesis and chemoselective ligations. One such chemoselective reaction suitable for protein synthesis is the α-Ketoacid-Hydroxylamine (KAHA) ligation. Fully unprotected peptides are ligated by a selective reaction between α-ketoacids and hydroxylamines to give native amide bonds. Herein, we describe the chemical synthesis of ubiquitin by a two-segment approach using the 5-oxaproline hydroxylamine.
Identifiants
pubmed: 34386958
doi: 10.1007/978-1-0716-1617-8_14
doi:
Substances chimiques
Hydroxylamines
0
Keto Acids
0
Proteins
0
Hydroxylamine
2FP81O2L9Z
5-oxaproline
36839-09-5
Proline
9DLQ4CIU6V
Types de publication
Journal Article
Langues
eng
Sous-ensembles de citation
IM
Pagination
151-162Informations de copyright
© 2021. The Author(s), under exclusive license to Springer Science+Business Media, LLC, part of Springer Nature.
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