SPF45/RBM17-dependent, but not U2AF-dependent, splicing in a distinct subset of human short introns.
Base Sequence
Binding Sites
/ genetics
Humans
Introns
/ genetics
Models, Genetic
Nuclear Proteins
/ genetics
Phosphoproteins
/ genetics
Protein Binding
RNA Precursors
/ genetics
RNA Splicing
RNA Splicing Factors
/ genetics
Ribonucleoprotein, U2 Small Nuclear
/ genetics
Spliceosomes
/ genetics
Splicing Factor U2AF
/ genetics
Journal
Nature communications
ISSN: 2041-1723
Titre abrégé: Nat Commun
Pays: England
ID NLM: 101528555
Informations de publication
Date de publication:
13 08 2021
13 08 2021
Historique:
received:
10
09
2020
accepted:
06
07
2021
entrez:
14
8
2021
pubmed:
15
8
2021
medline:
31
8
2021
Statut:
epublish
Résumé
Human pre-mRNA introns vary in size from under fifty to over a million nucleotides. We searched for essential factors involved in the splicing of human short introns by screening siRNAs against 154 human nuclear proteins. The splicing activity was assayed with a model HNRNPH1 pre-mRNA containing short 56-nucleotide intron. We identify a known alternative splicing regulator SPF45 (RBM17) as a constitutive splicing factor that is required to splice out this 56-nt intron. Whole-transcriptome sequencing of SPF45-deficient cells reveals that SPF45 is essential in the efficient splicing of many short introns. To initiate the spliceosome assembly on a short intron with the truncated poly-pyrimidine tract, the U2AF-homology motif (UHM) of SPF45 competes out that of U2AF
Identifiants
pubmed: 34389706
doi: 10.1038/s41467-021-24879-y
pii: 10.1038/s41467-021-24879-y
pmc: PMC8363638
doi:
Substances chimiques
Nuclear Proteins
0
Phosphoproteins
0
RBM17 protein, human
0
RNA Precursors
0
RNA Splicing Factors
0
Ribonucleoprotein, U2 Small Nuclear
0
SF3B1 protein, human
0
Splicing Factor U2AF
0
Types de publication
Journal Article
Research Support, Non-U.S. Gov't
Langues
eng
Sous-ensembles de citation
IM
Pagination
4910Informations de copyright
© 2021. The Author(s).
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