Phospholipase Dα6 and phosphatidic acid regulate gibberellin signaling in rice.
GID1 receptor
Phospholipase
gibberellin signaling
phosphatidic acid
rice
Journal
EMBO reports
ISSN: 1469-3178
Titre abrégé: EMBO Rep
Pays: England
ID NLM: 100963049
Informations de publication
Date de publication:
05 10 2021
05 10 2021
Historique:
revised:
07
06
2021
received:
15
10
2020
accepted:
22
07
2021
pubmed:
17
8
2021
medline:
8
10
2022
entrez:
16
8
2021
Statut:
ppublish
Résumé
Phospholipase D (PLD) hydrolyzes membrane lipids to produce phosphatidic acid (PA), a lipid mediator involved in various cellular and physiological processes. Here, we show that PLDα6 and PA regulate the distribution of GIBBERELLIN (GA)-INSENSITIVE DWARF1 (GID1), a soluble gibberellin receptor in rice. PLDα6-knockout (KO) plants display less sensitivity to GA than WT, and PA restores the mutant to a normal GA response. PA binds to GID1, as documented by liposome binding, fat immunoblotting, and surface plasmon resonance. Arginines 79 and 82 of GID1 are two key amino acid residues required for PA binding and also for GID1's nuclear localization. The loss of PLDα6 impedes GA-induced nuclear localization of GID1. In addition, PLDα6-KO plants attenuated GA-induced degradation of the DELLA protein SLENDER RICE1 (SLR1). These data suggest that PLDα6 and PA positively mediate GA signaling in rice via PA binding to GID1 and promotion of its nuclear translocation.
Identifiants
pubmed: 34396669
doi: 10.15252/embr.202051871
pmc: PMC8490989
doi:
Substances chimiques
Gibberellins
0
Phosphatidic Acids
0
Plant Growth Regulators
0
Plant Proteins
0
Phospholipases
EC 3.1.-
Types de publication
Journal Article
Research Support, Non-U.S. Gov't
Langues
eng
Sous-ensembles de citation
IM
Pagination
e51871Informations de copyright
© 2021 The Authors.
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