Spatially patterned excitatory neuron subtypes and projections of the claustrum.
Claustrum
cell type
mouse
neuroscience
transcriptome
Journal
eLife
ISSN: 2050-084X
Titre abrégé: Elife
Pays: England
ID NLM: 101579614
Informations de publication
Date de publication:
16 08 2021
16 08 2021
Historique:
received:
31
03
2021
accepted:
28
07
2021
entrez:
16
8
2021
pubmed:
17
8
2021
medline:
13
10
2021
Statut:
epublish
Résumé
The claustrum is a functionally and structurally complex brain region, whose very spatial extent remains debated. Histochemical-based approaches typically treat the claustrum as a relatively narrow anatomical region that primarily projects to the neocortex, whereas circuit-based approaches can suggest a broader claustrum region containing projections to the neocortex and other regions. Here, in the mouse, we took a bottom-up and cell-type-specific approach to complement and possibly unite these seemingly disparate conclusions. Using single-cell RNA-sequencing, we found that the claustrum comprises two excitatory neuron subtypes that are differentiable from the surrounding cortex. Multicolor retrograde tracing in conjunction with 12-channel multiplexed in situ hybridization revealed a core-shell spatial arrangement of these subtypes, as well as differential downstream targets. Thus, the claustrum comprises excitatory neuron subtypes with distinct molecular and projection properties, whose spatial patterns reflect the narrower and broader claustral extents debated in previous research. This subtype-specific heterogeneity likely shapes the functional complexity of the claustrum.
Identifiants
pubmed: 34397382
doi: 10.7554/eLife.68967
pii: 68967
pmc: PMC8367382
doi:
pii:
Banques de données
GEO
['GSE149495']
Types de publication
Journal Article
Research Support, Non-U.S. Gov't
Research Support, U.S. Gov't, Non-P.H.S.
Langues
eng
Sous-ensembles de citation
IM
Subventions
Organisme : CIHR
ID : PJT-419798
Pays : Canada
Organisme : Howard Hughes Medical Institute
Pays : United States
Informations de copyright
© 2021, Erwin et al.
Déclaration de conflit d'intérêts
SE, KS, RK, BM, LW, JC, AL, JJ, MC none, BB None
Références
J Comp Neurol. 2017 Apr 15;525(6):1363-1380
pubmed: 26860547
Elife. 2018 Oct 30;7:
pubmed: 30375971
Elife. 2020 Sep 01;9:
pubmed: 32869744
Cell. 2018 Aug 9;174(4):999-1014.e22
pubmed: 30096314
Nat Methods. 2012 Jun 28;9(7):676-82
pubmed: 22743772
Front Integr Neurosci. 2012 Aug 02;6:53
pubmed: 22876222
Philos Trans R Soc Lond B Biol Sci. 2005 Jun 29;360(1458):1271-9
pubmed: 16147522
Curr Biol. 2018 Sep 10;28(17):2752-2762.e7
pubmed: 30122531
J Comp Neurol. 2020 Dec 1;528(17):2956-2977
pubmed: 32266722
Neuroscience. 1985 Nov;16(3):557-76
pubmed: 4094690
J Neurosci. 2017 Jan 11;37(2):269-280
pubmed: 28077707
J Comp Neurol. 2021 May 1;529(7):1607-1627
pubmed: 32975316
Trends Neurosci. 2015 Aug;38(8):486-95
pubmed: 26116988
Nature. 2007 Jan 11;445(7124):168-76
pubmed: 17151600
Cell. 2018 Aug 9;174(4):1015-1030.e16
pubmed: 30096299
Neuron. 2016 Oct 19;92(2):372-382
pubmed: 27720486
Nat Methods. 2017 Mar;14(3):309-315
pubmed: 28114287
Front Neuroanat. 2019 Mar 26;13:34
pubmed: 30971901
J Comp Neurol. 2018 Oct 15;526(15):2428-2443
pubmed: 30252130
Front Syst Neurosci. 2014 Apr 04;8:48
pubmed: 24772070
Nature. 2020 Feb;578(7795):413-418
pubmed: 32051589
Cell. 2018 May 17;173(5):1280-1292.e18
pubmed: 29681453
Front Neuroanat. 2019 May 31;13:53
pubmed: 31213993
Neuron. 2018 Sep 5;99(5):1029-1039.e4
pubmed: 30122374
Cell. 2015 Jul 2;162(1):184-97
pubmed: 26095251
Nat Neurosci. 2016 Feb;19(2):335-46
pubmed: 26727548
J Comp Neurol. 2016 Feb 15;524(3):665-703
pubmed: 26400616
J Comp Neurol. 2012 Nov 1;520(16):3553-73
pubmed: 22678985
Nat Neurosci. 2020 Jun;23(6):741-753
pubmed: 32393895
J Comp Neurol. 2017 Apr 15;525(6):1317-1346
pubmed: 27223051
Cereb Cortex. 2009 Oct;19(10):2372-9
pubmed: 19168664
Bioinformatics. 2013 Jan 1;29(1):15-21
pubmed: 23104886
Nat Biotechnol. 2014 Apr;32(4):381-386
pubmed: 24658644
J Neurosci Methods. 2019 Oct 1;326:108353
pubmed: 31351971
Sci Rep. 2019 Mar 26;9(1):5233
pubmed: 30914743
Nature. 2019 Feb;566(7745):496-502
pubmed: 30787437
Cell. 2019 Jun 13;177(7):1888-1902.e21
pubmed: 31178118
Elife. 2020 Nov 03;9:
pubmed: 33141021
Cell Mol Biol (Noisy-le-grand). 2004 Sep;50(6):675-702
pubmed: 15643691
Phys Med Biol. 2010 Oct 21;55(20):6215-42
pubmed: 20885021
J Neurosci. 2019 Dec 11;39(50):10071-10080
pubmed: 31704786
J Neurosci. 2020 Apr 15;40(16):3250-3267
pubmed: 32198185
Nature. 2018 Nov;563(7729):72-78
pubmed: 30382198
Nat Protoc. 2007;2(11):2924-9
pubmed: 18007629
J Mol Diagn. 2012 Jan;14(1):22-9
pubmed: 22166544
Science. 2020 Oct 16;370(6514):
pubmed: 33060328
J Comp Neurol. 2019 Feb 1;527(2):476-499
pubmed: 30225888
Trends Neurosci. 2018 Jun;41(6):337-348
pubmed: 29576429
Sci Adv. 2015 Apr 03;1(3):e1400177
pubmed: 26601158
Nat Biotechnol. 2015 May;33(5):495-502
pubmed: 25867923