Isolation, characterization and comparison of lytic Epseptimavirus phages targeting Salmonella.
Food control
Foodborne pathogen
Genomic comparison
Genus Epseptimavirus
Phage
Salmonella
Taxonomy
Journal
Food research international (Ottawa, Ont.)
ISSN: 1873-7145
Titre abrégé: Food Res Int
Pays: Canada
ID NLM: 9210143
Informations de publication
Date de publication:
09 2021
09 2021
Historique:
received:
15
12
2020
revised:
17
05
2021
accepted:
23
05
2021
entrez:
17
8
2021
pubmed:
18
8
2021
medline:
7
9
2021
Statut:
ppublish
Résumé
This study describes the characterization and genomic analysis of six lytic Salmonella phages. To examine the feasibility of using these phages as biocontrol agents, we analyzed their genomes and compared them to those of similar phages. These six phages belong to genus Epseptimavirus, family Demerecviridae. We identified the genes of these six phages by comparing their genomes with those of three type phages in subfamily Markadamsvirinae. All six phages examined in this study were obligately lytic and did not carry undesirable genes. Two phages (vB_SalS_1-23 and vB_SalS_3-29) were selected as the representative phages for general characterization and physiological tests. The biocontrol efficacy of the representative phages was determined by comparing the viable counts of recovered host Salmonella ser. Newlands ZC-S1 from treatment and phage-free control samples. The biocontrol experiment showed that the representative phages were able to reduce the counts of ZC-S1 to below 2 log
Identifiants
pubmed: 34399476
pii: S0963-9969(21)00379-3
doi: 10.1016/j.foodres.2021.110480
pii:
doi:
Types de publication
Journal Article
Research Support, Non-U.S. Gov't
Langues
eng
Sous-ensembles de citation
IM
Pagination
110480Informations de copyright
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