Phosphorylation of PUF-A/PUM3 on Y259 modulates PUF-A stability and cell proliferation.
Atlases as Topic
CRISPR-Cas Systems
Carcinogenesis
/ genetics
Cell Movement
Cell Nucleolus
/ genetics
Cell Proliferation
Databases, Genetic
Female
Gene Deletion
HeLa Cells
Humans
Minor Histocompatibility Antigens
/ genetics
Mutation
Neoplasms
/ genetics
Phosphoproteins
/ genetics
Phosphorylation
Protein Processing, Post-Translational
Protein Stability
Survival Analysis
Tyrosine
/ metabolism
Journal
PloS one
ISSN: 1932-6203
Titre abrégé: PLoS One
Pays: United States
ID NLM: 101285081
Informations de publication
Date de publication:
2021
2021
Historique:
received:
09
04
2021
accepted:
03
08
2021
entrez:
18
8
2021
pubmed:
19
8
2021
medline:
15
12
2021
Statut:
epublish
Résumé
Human PUF-A/PUM3 is a RNA and DNA binding protein participating in the nucleolar processing of 7S to 5.8S rRNA. The nucleolar localization of PUF-A redistributes to the nucleoplasm upon the exposure to genotoxic agents in cells. However, little is known regarding the roles of PUF-A in tumor progression. Phosphoprotein database analysis revealed that Y259 phosphorylation of PUF-A is the most prevalent residue modified. Here, we reported the importance of PUF-A's phosphorylation on Y259 in tumorigenesis. PUF-A gene was knocked out by the Crispr/Cas9 method in human cervix epithelial HeLa cells. Loss of PUF-A in HeLa cells resulted in reduced clonogenic and lower transwell invasion capacity. Introduction of PUF-AY259F to PUF-A deficient HeLa cells was unable to restore colony formation. In addition, the unphosphorylated mutant of PUF-A, PUF-AY259F, attenuated PUF-A protein stability. Our results suggest the important role of Y259 phosphorylation of PUF-A in cell proliferation.
Identifiants
pubmed: 34407138
doi: 10.1371/journal.pone.0256282
pii: PONE-D-21-11723
pmc: PMC8372891
doi:
Substances chimiques
Minor Histocompatibility Antigens
0
PUM3 protein, human
0
Phosphoproteins
0
Tyrosine
42HK56048U
Types de publication
Journal Article
Research Support, Non-U.S. Gov't
Langues
eng
Sous-ensembles de citation
IM
Pagination
e0256282Déclaration de conflit d'intérêts
The authors have declared that no competing interests exist.
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