Cloning and expression analysis of mevalonate kinase and phosphomevalonate kinase genes associated with the MVA pathway in Santalum album.
Acetates
/ pharmacology
Amino Acid Sequence
Cloning, Molecular
Computational Biology
Cyclopentanes
/ pharmacology
DNA, Complementary
/ genetics
Evolution, Molecular
Gene Expression Profiling
Gene Expression Regulation, Plant
/ drug effects
Genes, Plant
Organ Specificity
/ drug effects
Oxylipins
/ pharmacology
Phosphotransferases (Alcohol Group Acceptor)
/ chemistry
Phosphotransferases (Phosphate Group Acceptor)
/ chemistry
Phylogeny
Plant Leaves
/ drug effects
Plant Proteins
/ chemistry
Plant Roots
/ drug effects
Plant Shoots
/ drug effects
Protein Domains
RNA, Messenger
/ genetics
Saccharomyces cerevisiae
/ metabolism
Santalum
/ drug effects
Subcellular Fractions
/ metabolism
Journal
Scientific reports
ISSN: 2045-2322
Titre abrégé: Sci Rep
Pays: England
ID NLM: 101563288
Informations de publication
Date de publication:
19 08 2021
19 08 2021
Historique:
received:
21
12
2020
accepted:
23
07
2021
entrez:
20
8
2021
pubmed:
21
8
2021
medline:
10
11
2021
Statut:
epublish
Résumé
Sandalwood (Santalum album L.) is highly valued for its fragrant heartwood and extracted oil. Santalols, which are the main components of that oil, are terpenoids, and these are biosynthesized via the mevalonic acid (MVA) pathway. Mevalonate kinase (MK) and phosphomevalonate kinase (PMK) are key enzymes in the MVA pathway. Little is known about the genes that encode MK and PMK in S. album or the mechanism that regulates their expression. To isolate and identify the functional genes involved in santalol biosynthesis in S. album, an MK gene designated as SaMK, and a PMK gene designated as SaPMK, were cloned from S. album. The sequences of these genes were analyzed. A bioinformatics analysis was conducted to assess the homology of SaMK and SaPMK with MK and PMK genes from other plants. The subcellular localization of SaMK and SaPMK proteins was also investigated, as was the functional complementation of SaMK and SaPMK in yeast. Our results show that the full-length cDNA sequences of SaMK and SaPMK were 1409 bp and 1679 bp long, respectively. SaMK contained a 1381 bp open reading frame (ORF) encoding a polypeptide of 460 amino acids and SaPMK contained a 1527 bp ORF encoding a polypeptide of 508 amino acids. SaMK and SaPMK showed high homology with MK and PMK genes of other plant species. Functional complementation of SaMK in a MK-deficient mutant yeast strain YMR208W and SaPMK in a PMK-deficient mutant yeast strain YMR220W confirmed that cloned SaMK and SaPMK cDNA encode a functional MK and PMK, respectively, mediating MVA biosynthesis in yeast. An analysis of tissue expression patterns revealed that SaMK and SaPMK were constitutively expressed in all the tested tissues. SaMK was highly expressed in young leaves but weakly expressed in sapwood. SaPMK was highly expressed in roots and mature leaves, but weakly expressed in young leaves. Induction experiments with several elicitors showed that SaMK and SaPMK expression was upregulated by methyl jasmonate. These results will help to further study the role of MK and PMK genes during santalol biosynthesis in S. album.
Identifiants
pubmed: 34413433
doi: 10.1038/s41598-021-96511-4
pii: 10.1038/s41598-021-96511-4
pmc: PMC8376994
doi:
Substances chimiques
Acetates
0
Cyclopentanes
0
DNA, Complementary
0
Oxylipins
0
Plant Proteins
0
RNA, Messenger
0
methyl jasmonate
900N171A0F
Phosphotransferases (Alcohol Group Acceptor)
EC 2.7.1.-
mevalonate kinase
EC 2.7.1.36
Phosphotransferases (Phosphate Group Acceptor)
EC 2.7.4.-
phosphomevalonate kinase
EC 2.7.4.2
Types de publication
Journal Article
Research Support, Non-U.S. Gov't
Langues
eng
Sous-ensembles de citation
IM
Pagination
16913Informations de copyright
© 2021. The Author(s).
Références
J Exp Bot. 2012 Apr;63(7):2809-23
pubmed: 22291132
Chem Biodivers. 2008 Jun;5(6):1000-10
pubmed: 18618410
Gene. 2013 Sep 25;527(2):642-8
pubmed: 23860319
Nat Protoc. 2008;3(6):1101-8
pubmed: 18546601
Proc Natl Acad Sci U S A. 2008 Jan 29;105(4):1380-5
pubmed: 18216250
Sci Rep. 2021 Jan 13;11(1):1082
pubmed: 33441887
Annu Rev Phytopathol. 2011;49:317-43
pubmed: 21663438
Annu Rev Plant Physiol Plant Mol Biol. 1999 Jun;50:47-65
pubmed: 15012203
FEBS Lett. 2010 Jul 16;584(14):2965-73
pubmed: 20553718
Metab Eng. 2011 Mar;13(2):194-203
pubmed: 21215324
Phytomedicine. 2013 Mar 15;20(5):409-16
pubmed: 23369343
J Biol Chem. 1968 Sep 25;243(18):4667-70
pubmed: 4300840
Curr Genet. 1991 Jan;19(1):9-14
pubmed: 1645230
Arch Biochem Biophys. 2000 Jun 15;378(2):287-98
pubmed: 10860546
Proteins. 2002 Jun 1;47(4):568-71
pubmed: 12001237
Plant Mol Biol. 2009 Jan;69(1-2):179-94
pubmed: 18937034
Nat Prod Res. 2019 Feb;33(4):527-543
pubmed: 29130352
Mol Biol Evol. 1987 Jul;4(4):406-25
pubmed: 3447015
Arch Biochem Biophys. 2008 Sep 1;477(1):121-30
pubmed: 18541135
Nat Protoc. 2007;2(7):1565-72
pubmed: 17585298
BMC Plant Biol. 2019 Mar 28;19(1):115
pubmed: 30922222
Proc Natl Acad Sci U S A. 2003 May 27;100(11):6866-71
pubmed: 12748386
Org Lett. 2009 Nov 19;11(22):5170-3
pubmed: 19905026
Biologist (London). 2000 Feb;47(1):31-4
pubmed: 11190217
Biosci Biotechnol Biochem. 2002 Aug;66(8):1619-27
pubmed: 12353619
Molecules. 2017 Dec 10;22(12):
pubmed: 29232862
Gene. 1994 Oct 21;148(2):293-7
pubmed: 7958957
Protein Expr Purif. 2003 Aug;30(2):210-8
pubmed: 12880770
PLoS One. 2014 Jan 27;9(1):e87112
pubmed: 24475236
Trends Genet. 2003 Jul;19(7):409-13
pubmed: 12850447
J Chromatogr A. 2004 Mar 5;1028(2):307-12
pubmed: 14989484
Plant Mol Biol. 2000 Jan;42(2):365-76
pubmed: 10794536
Plants (Basel). 2021 Feb 10;10(2):
pubmed: 33578821
Plant J. 2016 May;86(4):289-99
pubmed: 26991058
J Ind Microbiol Biotechnol. 2009 Aug;36(8):1085-91
pubmed: 19462190
Biochem J. 1965 Sep;96(3):862-71
pubmed: 5862423
Front Plant Sci. 2015 Sep 01;6:661
pubmed: 26388878
Planta. 2016 Apr;243(4):847-87
pubmed: 26745967
Nat Chem Biol. 2006 Dec;2(12):674-81
pubmed: 17108985
J Biosci. 2003 Sep;28(5):637-46
pubmed: 14517367
J Plant Growth Regul. 2016 Dec;35(4):921-935
pubmed: 29367803
Phytochemistry. 2009 Oct-Nov;70(15-16):1621-37
pubmed: 19793600
Science. 2010 Oct 1;330(6000):70-4
pubmed: 20929806
Food Chem Toxicol. 2008 Feb;46(2):421-32
pubmed: 17980948
PLoS One. 2013 Sep 18;8(9):e75053
pubmed: 24324844
Mol Cell Biol. 1991 Feb;11(2):620-31
pubmed: 1846667
Molecules. 2017 Jan 02;22(1):
pubmed: 28045448
Biochem J. 1986 Dec 1;240(2):541-7
pubmed: 2880580
Plant J. 2013 Aug;75(3):351-63
pubmed: 23607888
Biosci Biotechnol Biochem. 2008 Aug;72(8):2049-60
pubmed: 18685207
Sci Rep. 2015 May 15;5:10095
pubmed: 25976282
Protein Sci. 2005 May;14(5):1134-9
pubmed: 15802646
J Biol Chem. 2011 May 20;286(20):17445-54
pubmed: 21454632
Planta. 2011 Nov;234(5):903-14
pubmed: 21655959