The phosphorylated pathway of serine biosynthesis is crucial for indolic glucosinolate biosynthesis and plant growth promotion conferred by the root endophyte Colletotrichum tofieldiae.
Amino Acids
/ metabolism
Arabidopsis
/ genetics
Biosynthetic Pathways
Colletotrichum
/ physiology
Endophytes
/ physiology
Gene Expression Regulation, Plant
Genes, Plant
Glucosinolates
/ biosynthesis
Indoles
/ metabolism
Phosphorylation
Plant Development
Plant Roots
/ microbiology
Serine
/ biosynthesis
Stress, Physiological
/ genetics
Transcription Factors
/ metabolism
Tryptophan
/ biosynthesis
Indolic glucosinolate Biosynthesis
Plant growth promotion
Serine biosynthesis
Journal
Plant molecular biology
ISSN: 1573-5028
Titre abrégé: Plant Mol Biol
Pays: Netherlands
ID NLM: 9106343
Informations de publication
Date de publication:
Sep 2021
Sep 2021
Historique:
received:
03
03
2021
accepted:
15
08
2021
pubmed:
24
8
2021
medline:
23
9
2021
entrez:
23
8
2021
Statut:
ppublish
Résumé
Phosphoglycerate Dehydrogenase 1 of the phosphorylated pathway of serine biosynthesis, active in heterotrophic plastids, is required for the synthesis of serine to enable plant growth at high rates of indolic glucosinolate biosynthesis. Plants have evolved effective strategies to defend against various types of pathogens. The synthesis of a multitude of specialized metabolites represents one effective approach to keep plant attackers in check. The synthesis of those defense compounds is cost intensive and requires extensive interaction with primary metabolism. However, how primary metabolism is adjusted to fulfill the requirements of specialized metabolism is still not completely resolved. Here, we studied the role of the phosphorylated pathway of serine biosynthesis (PPSB) for the synthesis of glucosinolates, the main class of defensive compounds in the model plant Arabidopsis thaliana. We show that major genes of the PPSB are co-expressed with genes required for the synthesis of tryptophan, the unique precursor for the formation of indolic glucosinolates (IG). Transcriptional and metabolic characterization of loss-of-function and dominant mutants of ALTERED TRYPTOPHAN1-like transcription factors revealed demand driven activation of PPSB genes by major regulators of IG biosynthesis. Trans-activation of PPSB promoters by ATR1/MYB34 transcription factor in cultured root cells confirmed this finding. The content of IGs were significantly reduced in plants compromised in the PPSB and these plants showed higher sensitivity against treatment with 5-methyl-tryptophan, a characteristic behavior of mutants impaired in IG biosynthesis. We further found that serine produced by the PPSB is required to enable plant growth under conditions of high demand for IG. In addition, PPSB-deficient plants lack the growth promoting effect resulting from interaction with the beneficial root-colonizing fungus Colletotrichum tofieldiae.
Identifiants
pubmed: 34424501
doi: 10.1007/s11103-021-01181-5
pii: 10.1007/s11103-021-01181-5
pmc: PMC8443527
doi:
Substances chimiques
Amino Acids
0
Glucosinolates
0
Indoles
0
Transcription Factors
0
Serine
452VLY9402
Tryptophan
8DUH1N11BX
Types de publication
Journal Article
Langues
eng
Sous-ensembles de citation
IM
Pagination
85-100Subventions
Organisme : Deutsche Forschungsgemeinschaft
ID : Kr4245/2-1
Organisme : Deutsche Forschungsgemeinschaft
ID : EXC-2048/1
Informations de copyright
© 2021. The Author(s).
Références
Annu Rev Plant Biol. 2017 Apr 28;68:513-534
pubmed: 28142282
Methods Mol Biol. 2017;1653:227-242
pubmed: 28822137
Plant Cell Environ. 2015 Jan;38(1):172-87
pubmed: 24894834
Proc Natl Acad Sci U S A. 2004 May 25;101(21):8245-50
pubmed: 15148388
Cell. 2016 Apr 7;165(2):464-74
pubmed: 26997485
Plant J. 2007 Jun;50(5):911-6
pubmed: 17425717
Nat Commun. 2016 May 06;7:11362
pubmed: 27150427
Plant J. 2007 Jul;51(2):247-61
pubmed: 17521412
Plant Cell. 1998 Mar;10(3):359-70
pubmed: 9501110
Arabidopsis Book. 2010;8:e0134
pubmed: 22303260
Annu Rev Entomol. 2009;54:57-83
pubmed: 18811249
Plant Cell. 2013 Jun;25(6):2084-101
pubmed: 23771893
Plant Cell. 2007 Jul;19(7):2225-45
pubmed: 17616737
Trends Plant Sci. 2018 Jul;23(7):577-587
pubmed: 29753631
Front Plant Sci. 2019 Dec 06;10:1560
pubmed: 31867028
Plant Physiol. 2003 Jan;131(1):298-308
pubmed: 12529537
Trends Plant Sci. 2020 Jun;25(6):549-565
pubmed: 32407695
Anal Biochem. 1976 May 7;72:248-54
pubmed: 942051
Plant Physiol. 2005 Jan;137(1):253-62
pubmed: 15579661
Genetics. 2002 Aug;161(4):1685-96
pubmed: 12196411
Mol Plant. 2014 May;7(5):814-28
pubmed: 24431192
Proc Natl Acad Sci U S A. 2007 Apr 10;104(15):6478-83
pubmed: 17420480
Nat Genet. 2007 Aug;39(8):1033-7
pubmed: 17643101
Plant J. 2010 Apr 1;62(1):1-11
pubmed: 20042022
New Phytol. 2012 Oct;196(2):596-605
pubmed: 22943527
Trends Plant Sci. 2014 Sep;19(9):564-9
pubmed: 24999240
Plant Cell. 2004 Jul;16(7):1938-50
pubmed: 15208388
Eur J Biochem. 1993 Mar 1;212(2):431-40
pubmed: 8444181
Mol Plant Microbe Interact. 2021 May;34(5):560-570
pubmed: 33226310
Front Plant Sci. 2018 Nov 20;9:1712
pubmed: 30515188
Phytochemistry. 2009 Oct-Nov;70(15-16):1680-6
pubmed: 19698961
Plant Cell. 2013 Dec;25(12):5011-29
pubmed: 24368794
Plant J. 2007 Jun;50(5):886-901
pubmed: 17461791
Plant Cell. 2011 Feb;23(2):716-29
pubmed: 21317374
Plant Cell. 2008 Jul;20(7):1736-7
pubmed: 18664613
Biochemistry. 2001 Mar 27;40(12):3497-511
pubmed: 11297416
Plant Physiol. 2008 Dec;148(4):2021-49
pubmed: 18829985
Planta. 2000 Nov;211(6):855-63
pubmed: 11144271
J Biol Chem. 2000 Oct 27;275(43):33712-7
pubmed: 10922360
Plant Cell. 1993 Sep;5(9):1011-27
pubmed: 8400875
Science. 2009 Jan 2;323(5910):101-6
pubmed: 19095900
Plant Cell. 1992 Jun;4(6):721-33
pubmed: 1392592
Proc Natl Acad Sci U S A. 1998 May 12;95(10):5655-60
pubmed: 9576939
Genes Dev. 2001 Aug 15;15(16):2122-33
pubmed: 11511543
Plant Cell. 2009 Mar;21(3):985-99
pubmed: 19293369
Genes Dev. 2002 Dec 1;16(23):3100-12
pubmed: 12464638
Plant Physiol. 2001 Jun;126(2):849-60
pubmed: 11402212
Ann Bot. 2013 Jun;111(6):1021-58
pubmed: 23558912
Arch Microbiol. 1977 Dec 15;115(3):307-16
pubmed: 341826
Chem Rec. 2001;1(2):140-51
pubmed: 11893063
Plant Physiol. 2000 Jul;123(3):1109-19
pubmed: 10889260
Plant Cell. 2010 Jul;22(7):2429-43
pubmed: 20605856
Plant Physiol. 2013 Nov;163(3):1164-78
pubmed: 24058165
Annu Rev Plant Biol. 2006;57:303-33
pubmed: 16669764