Differential inflammatory responses of the native left and right ventricle associated with donor heart preservation.
Animals
Cold Temperature
/ adverse effects
Female
Heart Transplantation
/ methods
Heart Ventricles
/ drug effects
Humans
Inflammation Mediators
/ metabolism
Male
Mice
Mice, Inbred C57BL
Middle Aged
Myocardial Ischemia
/ metabolism
Organ Preservation Solutions
/ administration & dosage
Tissue Donors
Ventricular Dysfunction, Left
/ metabolism
Ventricular Dysfunction, Right
/ metabolism
contractile function
inflammation
ischemia
myocardial biology
transplantation
Journal
Physiological reports
ISSN: 2051-817X
Titre abrégé: Physiol Rep
Pays: United States
ID NLM: 101607800
Informations de publication
Date de publication:
09 2021
09 2021
Historique:
revised:
14
07
2021
received:
12
06
2021
accepted:
18
07
2021
entrez:
26
8
2021
pubmed:
27
8
2021
medline:
25
2
2022
Statut:
ppublish
Résumé
Dysfunction and inflammation of hearts subjected to cold ischemic preservation may differ between left and right ventricles, suggesting distinct strategies for amelioration. Explanted murine hearts subjected to cold ischemia for 0, 4, or 8 h in preservation solution were assessed for function during 60 min of warm perfusion and then analyzed for cell death and inflammation by immunohistochemistry and western blotting and total RNA sequencing. Increased cold ischemic times led to greater left ventricle (LV) dysfunction compared to right ventricle (RV). The LV experienced greater cell death assessed by TUNEL Mouse hearts subjected to cold ischemia showed time-dependent contractile dysfunction and increased cell death, inflammatory cytokine expression and inflammasome expression that are greater in the LV than RV. However, IL-6 protein elevations and altered transcriptional profiles were similar in both ventricles. Similar changes are observed in human hearts.
Sections du résumé
BACKGROUND
Dysfunction and inflammation of hearts subjected to cold ischemic preservation may differ between left and right ventricles, suggesting distinct strategies for amelioration.
METHODS AND RESULTS
Explanted murine hearts subjected to cold ischemia for 0, 4, or 8 h in preservation solution were assessed for function during 60 min of warm perfusion and then analyzed for cell death and inflammation by immunohistochemistry and western blotting and total RNA sequencing. Increased cold ischemic times led to greater left ventricle (LV) dysfunction compared to right ventricle (RV). The LV experienced greater cell death assessed by TUNEL
CONCLUSIONS
Mouse hearts subjected to cold ischemia showed time-dependent contractile dysfunction and increased cell death, inflammatory cytokine expression and inflammasome expression that are greater in the LV than RV. However, IL-6 protein elevations and altered transcriptional profiles were similar in both ventricles. Similar changes are observed in human hearts.
Identifiants
pubmed: 34435466
doi: 10.14814/phy2.15004
pmc: PMC8387788
doi:
Substances chimiques
Inflammation Mediators
0
Organ Preservation Solutions
0
Types de publication
Journal Article
Research Support, N.I.H., Extramural
Research Support, U.S. Gov't, Non-P.H.S.
Langues
eng
Sous-ensembles de citation
IM
Pagination
e15004Subventions
Organisme : NHLBI NIH HHS
ID : R01 HL139735
Pays : United States
Organisme : NIAID NIH HHS
ID : U01 AI132895
Pays : United States
Informations de copyright
© 2021 The Authors. Physiological Reports published by Wiley Periodicals LLC on behalf of The Physiological Society and the American Physiological Society.
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