Characterization of the SARS-CoV-2 Host Response in Primary Human Airway Epithelial Cells from Aged Individuals.
Aged
Aging
/ immunology
Bronchi
/ cytology
COVID-19
/ immunology
Cell Death
/ genetics
Cells, Cultured
Cellular Senescence
/ genetics
Cytokines
/ biosynthesis
Epithelial Cells
/ immunology
Female
Humans
Immunity, Innate
Inflammation
Interferons
/ biosynthesis
Male
RNA-Seq
Respiratory Mucosa
/ cytology
SARS-CoV-2
/ immunology
Signal Transduction
/ genetics
COVID-19
SARS-CoV-2
aging
airway epithelium
air–liquid interface
immune response
inflammation
innate immunity
Journal
Viruses
ISSN: 1999-4915
Titre abrégé: Viruses
Pays: Switzerland
ID NLM: 101509722
Informations de publication
Date de publication:
12 08 2021
12 08 2021
Historique:
received:
10
06
2021
revised:
03
08
2021
accepted:
06
08
2021
entrez:
28
8
2021
pubmed:
29
8
2021
medline:
14
9
2021
Statut:
epublish
Résumé
Severe Acute Respiratory Syndrome Coronavirus 2 (SARS-CoV-2) is the causative agent of coronavirus disease 2019 (COVID-19), a global pandemic characterized by an exaggerated immune response and respiratory illness. Age (>60 years) is a significant risk factor for developing severe COVID-19. To better understand the host response of the aged airway epithelium to SARS-CoV-2 infection, we performed an in vitro study using primary human bronchial epithelial cells from donors >67 years of age differentiated on an air-liquid interface culture. We demonstrate that SARS-CoV-2 infection leads to early induction of a proinflammatory response and a delayed interferon response. In addition, we observed changes in the genes and pathways associated with cell death and senescence throughout infection. In summary, our study provides new and important insights into the temporal kinetics of the airway epithelial innate immune response to SARS-CoV-2 in older individuals.
Identifiants
pubmed: 34452468
pii: v13081603
doi: 10.3390/v13081603
pmc: PMC8402710
pii:
doi:
Substances chimiques
Cytokines
0
Interferons
9008-11-1
Types de publication
Journal Article
Research Support, N.I.H., Extramural
Research Support, Non-U.S. Gov't
Langues
eng
Sous-ensembles de citation
IM
Subventions
Organisme : NIGMS NIH HHS
ID : P20 GM103636
Pays : United States
Organisme : Presbyterian Health Foundation
ID : COVID-19 Seed Grant
Organisme : University of Oklahoma Health Sciences Center, College of Medicine
ID : COVID-19 Seed Grant
Organisme : NIH/NIGMS COBRE
ID : GM103636, Project 4
Références
Am J Respir Crit Care Med. 2020 Jul 15;202(2):219-229
pubmed: 32432483
Lancet Respir Med. 2020 Apr;8(4):420-422
pubmed: 32085846
Mol Cell. 2020 May 21;78(4):779-784.e5
pubmed: 32362314
Lancet. 2020 Mar 28;395(10229):1054-1062
pubmed: 32171076
Cell. 2020 May 28;181(5):1016-1035.e19
pubmed: 32413319
Bioinformatics. 2021 Apr 19;37(2):192-201
pubmed: 32730587
Cell Stem Cell. 2020 Dec 3;27(6):869-875.e4
pubmed: 33259798
Epigenetics. 2021 Mar;16(3):263-270
pubmed: 32686577
Immunity. 2020 Jul 14;53(1):19-25
pubmed: 32610079
Viruses. 2021 Apr 28;13(5):
pubmed: 33925255
Proc Natl Acad Sci U S A. 2020 May 26;117(21):11727-11734
pubmed: 32376634
Nat Commun. 2020 Nov 17;11(1):5854
pubmed: 33203890
EMBO J. 2020 May 18;39(10):e105114
pubmed: 32246845
mBio. 2021 Feb 9;12(1):
pubmed: 33563817
Nature. 2021 Jan;589(7841):270-275
pubmed: 33116299
Bioinformatics. 2015 Jan 15;31(2):166-9
pubmed: 25260700
Lancet Respir Med. 2020 Jul;8(7):687-695
pubmed: 32386571
Cell Stem Cell. 2020 Dec 3;27(6):905-919.e10
pubmed: 33142113
Diabetes. 2020 Sep;69(9):1857-1863
pubmed: 32669390
Nat Biomed Eng. 2021 Aug;5(8):815-829
pubmed: 33941899
Am J Physiol Lung Cell Mol Physiol. 2019 May 1;316(5):L826-L842
pubmed: 30785345
Protein J. 2020 Dec;39(6):644-656
pubmed: 33106987
Ann Am Thorac Soc. 2016 Dec;13 Suppl 5:S402-S406
pubmed: 28005423
Front Physiol. 2021 Jan 12;11:571416
pubmed: 33510644
Eur Respir J. 2020 Dec 24;56(6):
pubmed: 33033152
Nat Rev Microbiol. 2021 Mar;19(3):155-170
pubmed: 33116300
Front Cell Infect Microbiol. 2021 May 26;11:654813
pubmed: 34123871
Cell. 2020 Apr 16;181(2):281-292.e6
pubmed: 32155444
Cell Stem Cell. 2020 Dec 3;27(6):890-904.e8
pubmed: 33128895
Clin Immunol. 2021 Mar;224:108651
pubmed: 33333255
J Virol. 2020 Sep 15;94(19):
pubmed: 32699094
Am J Respir Crit Care Med. 2020 Dec 1;202(11):1509-1519
pubmed: 32866033
Cell. 2020 Jul 23;182(2):429-446.e14
pubmed: 32526206
Nat Commun. 2020 Oct 12;11(1):5139
pubmed: 33046696
Am J Respir Cell Mol Biol. 2021 Apr;64(4):426-440
pubmed: 33444514
Nature. 2021 Jul;595(7865):114-119
pubmed: 33915568
Genome Biol. 2014 Feb 03;15(2):R29
pubmed: 24485249
Cell Rep. 2021 May 4;35(5):109055
pubmed: 33905739
Nat Commun. 2020 Aug 6;11(1):3910
pubmed: 32764693
JAMA Intern Med. 2021 May 1;181(5):709-711
pubmed: 33492361
Bioinformatics. 2013 Jan 1;29(1):15-21
pubmed: 23104886
Biology (Basel). 2020 Dec 09;9(12):
pubmed: 33317104
PLoS Biol. 2021 Mar 17;19(3):e3001143
pubmed: 33730024
Cell Host Microbe. 2020 Mar 11;27(3):325-328
pubmed: 32035028
Cell. 2020 May 28;181(5):1036-1045.e9
pubmed: 32416070
JAMA Intern Med. 2020 Jul 1;180(7):934-943
pubmed: 32167524
Nat Commun. 2020 Jul 30;11(1):3810
pubmed: 32733001
Signal Transduct Target Ther. 2020 Aug 14;5(1):156
pubmed: 32796814
Cell Rep. 2020 Jul 21;32(3):107940
pubmed: 32668216
Nature. 2020 Dec;588(7839):670-675
pubmed: 33238290
Signal Transduct Target Ther. 2020 Oct 9;5(1):235
pubmed: 33037188
Am J Respir Cell Mol Biol. 2019 Jul;61(1):21-30
pubmed: 30965013
Nature. 2021 Jul;595(7865):107-113
pubmed: 33915569
Cell. 2020 Apr 16;181(2):271-280.e8
pubmed: 32142651
Ageing Res Rev. 2021 Jan;65:101205
pubmed: 33137510
mBio. 2020 Nov 6;11(6):
pubmed: 33158999
J Clin Invest. 2018 Apr 2;128(4):1238-1246
pubmed: 29608137
PLoS Biol. 2021 Mar 29;19(3):e3001158
pubmed: 33780434
Am J Respir Crit Care Med. 2020 Sep 15;202(6):812-821
pubmed: 32584597
Nat Med. 2020 May;26(5):681-687
pubmed: 32327758
Aging Cell. 2020 Oct;19(10):e13237
pubmed: 32955770
Genome. 2021 Apr;64(4):372-385
pubmed: 33395363