First derivative synchronous fluorometric method to continuously measure monophenolase activity.

Monophenol Monooxygenase / metabolism Oxidation-Reduction Oxidoreductases / metabolism Tyrosine / metabolism
Borate First derivative synchronous fluorescence Inhibitor Monophenolase Tyrosine

Journal

Enzyme and microbial technology
ISSN: 1879-0909
Titre abrégé: Enzyme Microb Technol
Pays: United States
ID NLM: 8003761

Informations de publication

Date de publication:
Oct 2021
Historique:
received: 22 02 2021
revised: 24 07 2021
accepted: 27 07 2021
entrez: 7 9 2021
pubmed: 8 9 2021
medline: 9 9 2021
Statut: ppublish

Résumé

Tyrosinase plays an essential role in melanin biosynthesis and inherently exhibits both monophenolase and diphenolase activity. A first derivative synchronous fluorometric assay was established for directly monitoring monophenolase activity. The zero-crossing point at 322 nm for the first-derivative under synchronous fluorescence with Δλ = 67 nm was utilized to selectively quantify tyrosine in the presence of the reaction product dihydroxyphenylalanine (DOPA). The limit of detection (LOD) for tyrosine was 0.54 μM. The fluorescence intensity of tyrosine was monitored at intervals of 30 s to establish the time course of tyrosine consumption. The LOD for the monophenolase activity was 0.0706 U⋅ mL

Identifiants

DOI: 10.1016/j.enzmictec.2021.109884 PMID: 34489037
pubmed: 34489037
pii: S0141-0229(21)00142-3
doi: 10.1016/j.enzmictec.2021.109884
pii:
doi:

Substances chimiques

Tyrosine 42HK56048U
Oxidoreductases EC 1.-
monophenolase EC 1.14.18.-
Monophenol Monooxygenase EC 1.14.18.1

Types de publication

Journal Article

Langues

eng

Sous-ensembles de citation

IM

Pagination

109884

Informations de copyright

Copyright © 2021 Elsevier Inc. All rights reserved.

Auteurs

Ling Zhang (L)

Department of Pharmaceutical and Biological Engineering, School of Chemical Engineering, Sichuan University, Chengdu, 610065, China.

Jiaze Li (J)

Department of Pharmaceutical and Biological Engineering, School of Chemical Engineering, Sichuan University, Chengdu, 610065, China.

Xiawen Wang (X)

Department of Pharmaceutical and Biological Engineering, School of Chemical Engineering, Sichuan University, Chengdu, 610065, China.

Zhaoqi Ran (Z)

Department of Pharmaceutical and Biological Engineering, School of Chemical Engineering, Sichuan University, Chengdu, 610065, China.

Qi Shang (Q)

Department of Pharmaceutical and Biological Engineering, School of Chemical Engineering, Sichuan University, Chengdu, 610065, China.

Chan Chen (C)

Department of Pharmaceutical and Biological Engineering, School of Chemical Engineering, Sichuan University, Chengdu, 610065, China.

Weikang Tang (W)

Department of Pharmaceutical and Biological Engineering, School of Chemical Engineering, Sichuan University, Chengdu, 610065, China.

Wenbin Liu (W)

Department of Pharmaceutical and Biological Engineering, School of Chemical Engineering, Sichuan University, Chengdu, 610065, China. Electronic address: wbliu@scu.edu.cn.

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Classifications MeSH

questionsmedicales.fr › Enzymes et coenzymes › Enzymes › Oxidoreductases › Oxygénases › Mixed function oxygenases › Catechol oxidase › Monophenol monooxygenase › First derivative synchronous fluorometric...
questionsmedicales.fr › Métabolisme › Métabolisme énergétique › Oxydoréduction › First derivative synchronous fluorometric...
questionsmedicales.fr › Enzymes et coenzymes › Enzymes › Oxidoreductases › First derivative synchronous fluorometric...
questionsmedicales.fr › Acides aminés, peptides et protéines › Acides aminés › Acides aminés cycliques › Acides aminés aromatiques › Tyrosine › First derivative synchronous fluorometric...