Genetic analysis of Curcuma species from Asia based on intron regions of genes encoding diketide-CoA synthase and curcumin synthase.
Curcuma
Curcumin synthase
Diketide-CoA synthase
Genetic analysis
Intron length polymorphism
Subcloning
Journal
Journal of natural medicines
ISSN: 1861-0293
Titre abrégé: J Nat Med
Pays: Japan
ID NLM: 101518405
Informations de publication
Date de publication:
Jan 2022
Jan 2022
Historique:
received:
22
07
2021
accepted:
01
09
2021
pubmed:
9
9
2021
medline:
8
1
2022
entrez:
8
9
2021
Statut:
ppublish
Résumé
Intron length polymorphism (ILP) markers in genes encoding diketide-CoA synthase (DCS) and curcumin synthase (CURS) showed high identification rates in 13 Curcuma species from Asia. However, the sequences of the intron regions have not yet been analyzed. To elucidate the sequence differences in intron regions of the DCS and CURS genes and to search for specific sequences suitable for the identification of Curcuma species, a large number of sequences were determined through subcloning coupled with sequencing analysis of six Curcuma plant specimens belonging to five species that showed distinct ILP patterns. More than 30 sequences of each region from each specimen were grouped into genes DCS1, DCS2, or CURS1-3 and subsequently the sequences of the same genes were compared. Sequences belonging to the same gene showed inter-species similarity, and thus, these intron sequences were less informative within each single-gene region. The determined sequences from each specimen showed 3-5 kinds of sequence lengths in DCS intron I region, and 5-7 kinds of sequence lengths in CURS intron region. The length of determined sequences and the fragment number in each intron region were different among species, or specimens in C. longa, which were in accordance with the fragment lengths and numbers in their corresponding ILP patterns.
Identifiants
pubmed: 34495455
doi: 10.1007/s11418-021-01563-5
pii: 10.1007/s11418-021-01563-5
doi:
Substances chimiques
Curcumin
IT942ZTH98
Coenzyme A
SAA04E81UX
Types de publication
Journal Article
Langues
eng
Sous-ensembles de citation
IM
Pagination
276-280Subventions
Organisme : japan society for the promotion of science
ID : JP18K06714
Informations de copyright
© 2021. The Japanese Society of Pharmacognosy.
Références
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