Molecular epidemiology of bla
Escherichia coli Infections
/ diagnosis
Humans
Iran
/ epidemiology
Kidney Transplantation
/ adverse effects
Molecular Epidemiology
Multilocus Sequence Typing
Phylogeny
Urinary Tract Infections
/ diagnosis
Uropathogenic Escherichia coli
/ enzymology
Virulence
Virulence Factors
beta-Lactamases
/ genetics
CTX-M
MLST
Phylogenetic group
Uropathogenic Escherichia coli
Virulence factor
Journal
Annals of clinical microbiology and antimicrobials
ISSN: 1476-0711
Titre abrégé: Ann Clin Microbiol Antimicrob
Pays: England
ID NLM: 101152152
Informations de publication
Date de publication:
08 Sep 2021
08 Sep 2021
Historique:
received:
07
11
2020
accepted:
31
08
2021
entrez:
9
9
2021
pubmed:
10
9
2021
medline:
15
1
2022
Statut:
epublish
Résumé
This study aimed to investigate the phylogenetic characterization and virulence traits of uropathogenic Escherichia coli (UPEC) isolated from kidney transplant patients (KTPs) as well as non-KTPs and analyze the clonal distribution of Extended spectrum β-lactamases (ESBLs)-producing UPEC containing bla To this end, we determined virulence marker and the phylogenetic characterization of UPEC in non-KTPs (n = 65) and KTPs (n = 46). The non-KTPs were considered the control group of the study. Also, according to the Achtman scheme, we performed multilocus sequence typing to assess the relationship between twenty-nine of ESBL-producing isolates containing bla According to the results of PCR assay, the prevalence of virulence factor genes ranged from 0% (cnf and papG III) to 93.7% (fimH). Also, KTP isolates significantly differed from non-KTP isolates only in terms of the prevalence of pap GI elements. Moreover, the most frequent UPEC isolates were in phylogenetic group B The results further revealed significant differences between the UPEC isolates from KTPs and non-KTPs regarding the phylogroups C and PAI gene. Based on MLST analysis, we also observed a relatively high diversity in UPEC isolates obtained from KTPs and non-KTPs. Moreover, clonal complex (CC) 131 and ST131 were found to be the most prevalent clones and ST types, respectively. Besides, for the first time, ST8503 were reported in KTPs. These results suggested regular studies on characterization of UPEC isolates among KTPs.
Sections du résumé
BACKGROUND
BACKGROUND
This study aimed to investigate the phylogenetic characterization and virulence traits of uropathogenic Escherichia coli (UPEC) isolated from kidney transplant patients (KTPs) as well as non-KTPs and analyze the clonal distribution of Extended spectrum β-lactamases (ESBLs)-producing UPEC containing bla
METHODS
METHODS
To this end, we determined virulence marker and the phylogenetic characterization of UPEC in non-KTPs (n = 65) and KTPs (n = 46). The non-KTPs were considered the control group of the study. Also, according to the Achtman scheme, we performed multilocus sequence typing to assess the relationship between twenty-nine of ESBL-producing isolates containing bla
RESULTS
RESULTS
According to the results of PCR assay, the prevalence of virulence factor genes ranged from 0% (cnf and papG III) to 93.7% (fimH). Also, KTP isolates significantly differed from non-KTP isolates only in terms of the prevalence of pap GI elements. Moreover, the most frequent UPEC isolates were in phylogenetic group B
CONCLUSIONS
CONCLUSIONS
The results further revealed significant differences between the UPEC isolates from KTPs and non-KTPs regarding the phylogroups C and PAI gene. Based on MLST analysis, we also observed a relatively high diversity in UPEC isolates obtained from KTPs and non-KTPs. Moreover, clonal complex (CC) 131 and ST131 were found to be the most prevalent clones and ST types, respectively. Besides, for the first time, ST8503 were reported in KTPs. These results suggested regular studies on characterization of UPEC isolates among KTPs.
Identifiants
pubmed: 34496873
doi: 10.1186/s12941-021-00470-7
pii: 10.1186/s12941-021-00470-7
pmc: PMC8424993
doi:
Substances chimiques
Virulence Factors
0
beta-Lactamases
EC 3.5.2.6
Types de publication
Journal Article
Langues
eng
Sous-ensembles de citation
IM
Pagination
65Informations de copyright
© 2021. The Author(s).
Références
Clin Infect Dis. 2012 Sep;55(5):712-9
pubmed: 22615330
Nephrol Dial Transplant. 2019 May 1;34(5):878-885
pubmed: 30304506
J Infect Dev Ctries. 2017 Jan 30;11(1):51-57
pubmed: 28141590
Microb Drug Resist. 2020 Jan;26(1):60-70
pubmed: 31526226
Microb Drug Resist. 2020 Nov;26(11):1357-1364
pubmed: 32380906
Microb Drug Resist. 2019 Apr;25(3):386-393
pubmed: 30676258
Antimicrob Agents Chemother. 2003 Dec;47(12):3724-32
pubmed: 14638473
J Antimicrob Chemother. 2016 Aug;71(8):2125-30
pubmed: 27494832
J Antimicrob Chemother. 2015 Oct;70(10):2757-62
pubmed: 26183183
BMC Microbiol. 2019 May 30;19(1):117
pubmed: 31146674
PLoS One. 2019 Sep 12;14(9):e0222441
pubmed: 31513642
J Clin Med. 2019 Jul 07;8(7):
pubmed: 31284699
Clin Microbiol Rev. 2014 Jul;27(3):543-74
pubmed: 24982321
J Clin Microbiol. 2003 May;41(5):1929-35
pubmed: 12734229
Microb Pathog. 2017 Jun;107:276-279
pubmed: 28390979
PLoS One. 2012;7(9):e44238
pubmed: 22970186
Transplant Proc. 2010 Mar;42(2):483-5
pubmed: 20304171
J Clin Microbiol. 2005 Dec;43(12):5860-4
pubmed: 16333067
PLoS One. 2014 Jul 25;9(7):e101547
pubmed: 25061819
Infect Drug Resist. 2020 May 15;13:1429-1437
pubmed: 32523361
J Clin Microbiol. 2010 Aug;48(8):2709-14
pubmed: 20519468
Antimicrob Agents Chemother. 2018 Dec 21;63(1):
pubmed: 30348668
Enferm Infecc Microbiol Clin. 2015 Dec;33(10):679.e1-679.e21
pubmed: 25976754
Microbiologyopen. 2019 Jun;8(6):e00759
pubmed: 30358940
Front Microbiol. 2017 Dec 01;8:2334
pubmed: 29250044
Environ Microbiol Rep. 2013 Feb;5(1):58-65
pubmed: 23757131
J Infect. 2007 Jan;54(1):53-7
pubmed: 16533535
Infect Genet Evol. 2017 Nov;55:318-323
pubmed: 28987805
Nephrol Ther. 2017 Jun;13(4):236-244
pubmed: 28576434
Lett Appl Microbiol. 2016 Jan;62(1):84-90
pubmed: 26518617
Infect Drug Resist. 2019 Apr 17;12:893-903
pubmed: 31118697
Infect Drug Resist. 2019 Jul 09;12:1795-1803
pubmed: 31372004
J Antimicrob Chemother. 2012 Nov;67(11):2612-20
pubmed: 22843833
Antimicrob Resist Infect Control. 2018 Oct 3;7:118
pubmed: 30305891
Front Microbiol. 2017 Nov 21;8:2318
pubmed: 29209306
Appl Environ Microbiol. 2011 Nov;77(21):7620-32
pubmed: 21908635
J Glob Antimicrob Resist. 2018 Sep;14:118-125
pubmed: 29581075
J Glob Antimicrob Resist. 2018 Dec;15:74-78
pubmed: 29807202
Infect Drug Resist. 2019 Jul 17;12:2039-2047
pubmed: 31410031
J Chemother. 2016 Aug;28(4):343-5
pubmed: 25801185
Microbiol Spectr. 2016 Feb;4(1):
pubmed: 26999389
Microorganisms. 2019 Aug 10;7(8):
pubmed: 31405113
Bioinformatics. 2017 Jan 1;33(1):128-129
pubmed: 27605102
Infect Dis (Lond). 2015;47(10):714-8
pubmed: 26024285
PLoS One. 2015 Aug 03;10(8):e0134737
pubmed: 26237422
BMC Infect Dis. 2018 Nov 15;18(1):572
pubmed: 30442101
Int J Med Microbiol. 2013 Aug;303(6-7):305-17
pubmed: 23490927
J Clin Microbiol. 2007 Apr;45(4):1167-74
pubmed: 17287329