Evolutionarily conserved transcriptional landscape of the heart defining the chamber specific physiology.


Journal

Genomics
ISSN: 1089-8646
Titre abrégé: Genomics
Pays: United States
ID NLM: 8800135

Informations de publication

Date de publication:
11 2021
Historique:
received: 28 04 2021
revised: 17 08 2021
accepted: 05 09 2021
pubmed: 11 9 2021
medline: 1 4 2022
entrez: 10 9 2021
Statut: ppublish

Résumé

Cardiovascular disease (CVD) remains the leading cause of death worldwide. A deeper characterization of regional transcription patterns within different heart chambers may aid to improve our understanding of the molecular mechanisms involved in myocardial function and further, our ability to develop novel therapeutic strategies. Here, we used RNA sequencing to determine differentially expressed protein coding (PC) and long non-coding (lncRNA) transcripts within the heart chambers across seven vertebrate species and identified evolutionarily conserved chamber specific genes, lncRNAs and pathways. We investigated lncRNA homologs based on sequence, secondary structure, synteny and expressional conservation and found most lncRNAs to be conserved by synteny. Regional co-expression patterns of transcripts are modulated by multiple factors, including genomic overlap, strandedness and transcript biotype. Finally, we provide a community resource designated EvoACTG, which informs researchers on the conserved yet intertwined nature of the coding and non-coding cardiac transcriptome across popular model organisms in CVD research.

Identifiants

pubmed: 34506887
pii: S0888-7543(21)00343-8
doi: 10.1016/j.ygeno.2021.09.002
pii:
doi:

Substances chimiques

RNA, Long Noncoding 0

Types de publication

Journal Article Research Support, Non-U.S. Gov't

Langues

eng

Sous-ensembles de citation

IM

Pagination

3782-3792

Informations de copyright

Copyright © 2021 The Authors. Published by Elsevier Inc. All rights reserved.

Auteurs

Shrey Gandhi (S)

Institute of Human Genetics, Division of Genetic Epidemiology, University of Muenster, Muenster, Germany.

Anika Witten (A)

Institute of Human Genetics, Division of Genetic Epidemiology, University of Muenster, Muenster, Germany.

Federica De Majo (F)

Department of Molecular Genetics, Maastricht University, Maastricht, the Netherlands.

Martijn Gilbers (M)

Department of Cardiothoracic Surgery, CARIM School for Cardiovascular Diseases, Maastricht University Medical Centre+, Maastricht, the Netherlands.

Jos Maessen (J)

Department of Cardiothoracic Surgery, CARIM School for Cardiovascular Diseases, Maastricht University Medical Centre+, Maastricht, the Netherlands.

Ulrich Schotten (U)

Department of Physiology, CARIM School for Cardiovascular Diseases, Maastricht University, Maastricht, the Netherlands.

Leon J de Windt (LJ)

Department of Molecular Genetics, Maastricht University, Maastricht, the Netherlands.

Monika Stoll (M)

Institute of Human Genetics, Division of Genetic Epidemiology, University of Muenster, Muenster, Germany; Department of Biochemistry, Genetic Epidemiology and Statistical Genetics, CARIM School for Cardiovascular Diseases, Maastricht University, Maastricht, the Netherlands. Electronic address: mstoll@uni-muenster.de.

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Classifications MeSH