Microenvironmental effects can masquerade as substrate channelling in cascade biocatalysis.
Journal
Current opinion in biotechnology
ISSN: 1879-0429
Titre abrégé: Curr Opin Biotechnol
Pays: England
ID NLM: 9100492
Informations de publication
Date de publication:
02 2022
02 2022
Historique:
received:
13
07
2021
revised:
16
08
2021
accepted:
17
08
2021
pubmed:
15
9
2021
medline:
8
4
2022
entrez:
14
9
2021
Statut:
ppublish
Résumé
Natural cascades frequently use spatial organization to introduce beneficial substrate channeling mechanisms, a strategy that has been widely mimicked in many engineered multienzyme cascades with enhanced catalysis. Enabled by new molecular scaffolds it is now possible to test the effects of spatial organization on cascade kinetics; however, these scaffolds can also alter the microenvironment experienced by the assembled enzymes. We know from decades of enzyme immobilization research that the microenvironment affects enzymatic activity, thus complicating kinetic analysis. Here, we review these effects and discuss examples that exploit the microenvironment to improve single enzyme and cascade catalysis. In doing so, we highlight the challenges in ascribing kinetic enhancements directly to substrate channeling without first determining the effects of the microenvironment.
Identifiants
pubmed: 34521036
pii: S0958-1669(21)00160-9
doi: 10.1016/j.copbio.2021.08.014
pii:
doi:
Substances chimiques
Enzymes, Immobilized
0
Types de publication
Journal Article
Research Support, U.S. Gov't, Non-P.H.S.
Review
Langues
eng
Sous-ensembles de citation
IM
Pagination
233-239Informations de copyright
Copyright © 2021 Elsevier Ltd. All rights reserved.