DcCCD4 catalyzes the degradation of α-carotene and β-carotene to affect carotenoid accumulation and taproot color in carrot.
Daucus carota
CCD4
carotenoid-cleavage dioxygenases
carotenoids
taproot color
α-carotene
β-carotene
Journal
The Plant journal : for cell and molecular biology
ISSN: 1365-313X
Titre abrégé: Plant J
Pays: England
ID NLM: 9207397
Informations de publication
Date de publication:
11 2021
11 2021
Historique:
received:
09
04
2021
accepted:
20
08
2021
pubmed:
22
9
2021
medline:
27
1
2022
entrez:
21
9
2021
Statut:
ppublish
Résumé
Carotenoids are important natural pigments that give bright colors to plants. The difference in the accumulation of carotenoids is one of the key factors in the formation of various colors in carrot taproots. Carotenoid cleavage dioxygenases (CCDs), including CCD and 9-cis epoxycarotenoid dioxygenase, are the main enzymes involved in the cleavage of carotenoids in plants. Seven CCD genes have been annotated from the carrot genome. In this study, through expression analysis, we found that the expression level of DcCCD4 was significantly higher in the taproot of white carrot (low carotenoid content) than orange carrot (high carotenoid content). The overexpression of DcCCD4 in orange carrots caused the taproot color to be pale yellow, and the contents of α- and β-carotene decreased sharply. Mutant carrot with loss of DcCCD4 function exhibited yellow color (the taproot of the control carrot was white). The accumulation of β-carotene was also detected in taproot. Functional analysis of the DcCCD4 enzyme in vitro showed that it was able to cleave α- and β-carotene at the 9, 10 (9', 10') double bonds. In addition, the number of colored chromoplasts in the taproot cells of transgenic carrots overexpressing DcCCD4 was significantly reduced compared with that in normal orange carrots. Results showed that DcCCD4 affects the accumulation of carotenoids through cleavage of α- and β-carotene in carrot taproot.
Substances chimiques
Plant Proteins
0
beta Carotene
01YAE03M7J
Carotenoids
36-88-4
alpha-carotene
45XWE1Z69V
Dioxygenases
EC 1.13.11.-
9-cis-epoxy-carotenoid dioxygenase
EC 1.13.11.51
Types de publication
Journal Article
Research Support, Non-U.S. Gov't
Langues
eng
Sous-ensembles de citation
IM
Pagination
1116-1130Informations de copyright
© 2021 Society for Experimental Biology and John Wiley & Sons Ltd.
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