In Vitro Heme Coordination of a Dye-Decolorizing Peroxidase-The Interplay of Key Amino Acids, pH, Buffer and Glycerol.


Journal

International journal of molecular sciences
ISSN: 1422-0067
Titre abrégé: Int J Mol Sci
Pays: Switzerland
ID NLM: 101092791

Informations de publication

Date de publication:
12 Sep 2021
Historique:
received: 30 06 2021
revised: 31 08 2021
accepted: 03 09 2021
entrez: 28 9 2021
pubmed: 29 9 2021
medline: 25 2 2023
Statut: epublish

Résumé

Dye-decolorizing peroxidases (DyPs) have gained interest for their ability to oxidize anthraquinone-derived dyes and lignin model compounds. Spectroscopic techniques, such as electron paramagnetic resonance and optical absorption spectroscopy, provide main tools to study how the enzymatic function is linked to the heme-pocket architecture, provided the experimental conditions are carefully chosen. Here, these techniques are used to investigate the effect of active site perturbations on the structure of ferric P-class DyP from

Identifiants

pubmed: 34576013
pii: ijms22189849
doi: 10.3390/ijms22189849
pmc: PMC8468270
pii:
doi:

Substances chimiques

Amino Acids 0
Bacterial Proteins 0
Heme 42VZT0U6YR
Peroxidase EC 1.11.1.7
Glycerol PDC6A3C0OX

Types de publication

Evaluation Study Journal Article

Langues

eng

Sous-ensembles de citation

IM

Subventions

Organisme : Research Foundation Flanders / Austrian Science Fund - FWO/FWF lead agency grant
ID : G005416N

Références

Arch Biochem Biophys. 2015 May 15;574:108-19
pubmed: 25575902
J Biol Chem. 1966 Nov 25;241(22):5347-52
pubmed: 5954801
Biochem J. 2000 Nov 1;351 Pt 3:595-605
pubmed: 11042113
Biochemistry. 1989 Jun 13;28(12):5058-64
pubmed: 2548600
Biochim Biophys Acta. 1972 Sep 19;284(1):20-9
pubmed: 4342219
J Am Chem Soc. 2008 Feb 20;130(7):2128-9
pubmed: 18217756
J Am Chem Soc. 2004 Apr 14;126(14):4516-7
pubmed: 15070359
Biochim Biophys Acta. 1981 Sep 29;670(2):170-5
pubmed: 6271239
Inorg Chem. 2008 Dec 1;47(23):11294-304
pubmed: 19228028
Biochim Biophys Acta Proteins Proteom. 2021 Jan;1869(1):140536
pubmed: 32891739
Biochem J. 2015 Mar 1;466(2):253-62
pubmed: 25495127
J Biol Chem. 1987 Jul 15;262(20):9547-54
pubmed: 3036864
Biotechnol J. 2014 Apr;9(4):461-73
pubmed: 24519858
Eur Biophys J. 1993;22(4):259-67
pubmed: 8253054
Biochimie. 2012 Jun;94(6):1274-80
pubmed: 22381358
Biochemistry. 1997 Feb 11;36(6):1532-43
pubmed: 9063902
J Mol Biol. 2011 May 6;408(3):379-98
pubmed: 21354424
J Biol Chem. 1971 May 25;246(10):3342-55
pubmed: 4324897
Acc Chem Res. 2005 May;38(5):433-40
pubmed: 15895981
J Biol Chem. 1968 Apr 25;243(8):1854-62
pubmed: 5646479
Int J Mol Sci. 2021 May 24;22(11):
pubmed: 34074047
J Biol Chem. 1985 Jan 10;260(1):349-55
pubmed: 2981209
Biotechnol Bioeng. 2003 Jun 20;82(6):684-90
pubmed: 12673768
Biophys J. 2007 Feb 15;92(4):1361-73
pubmed: 17142277
J Biol Chem. 1988 Sep 15;263(26):13032-8
pubmed: 3417650
Arch Biochem Biophys. 2015 May 15;574:49-55
pubmed: 25655348
PLoS Comput Biol. 2012;8(10):e1002708
pubmed: 23093919
Biochem J. 1999 Nov 15;344 Pt 1:237-44
pubmed: 10548556
J Biol Chem. 2015 Apr 17;290(16):10336-52
pubmed: 25666609
Eur J Biochem. 1998 Feb 1;251(3):830-8
pubmed: 9490058
DNA Cell Biol. 2002 Apr;21(4):271-80
pubmed: 12042067
Biochemistry. 2014 Jul 29;53(29):4869-79
pubmed: 24988286
Biochemistry. 1996 Jun 18;35(24):7626-30
pubmed: 8672462
J Biol Inorg Chem. 2000 Apr;5(2):227-35
pubmed: 10819468
J Phys Chem B. 2008 Mar 27;112(12):3859-70
pubmed: 18321089
J Biol Chem. 2020 Sep 25;295(39):13488-13501
pubmed: 32723869
J Inorg Biochem. 2019 Oct;199:110761
pubmed: 31325671
Biochemistry. 2021 May 3;:
pubmed: 33939915
J Magn Reson. 2006 Jan;178(1):42-55
pubmed: 16188474
Phys Chem Chem Phys. 2010 Oct 21;12(39):12840-50
pubmed: 20820555
J Biol Chem. 1990 Sep 25;265(27):16043-53
pubmed: 2398044
Biochem J. 1977 Dec 1;167(3):593-600
pubmed: 23760
Biochemistry. 2012 Nov 27;51(47):9501-12
pubmed: 23126649
Biochemistry. 1979 Jul 10;18(14):2935-41
pubmed: 37888
Inorg Chem. 2006 Jul 10;45(14):5417-28
pubmed: 16813405
J Magn Reson. 1998 Apr;131(2):261-71
pubmed: 9571102
Biochim Biophys Acta. 2013 Sep;1834(9):1901-9
pubmed: 23467007
J Biol Chem. 1980 May 25;255(10):4801-7
pubmed: 6246083
J Biol Chem. 2018 Sep 21;293(38):14823-14838
pubmed: 30072383

Auteurs

Kevin Nys (K)

BIMEF Laboratory, Department of Chemistry, University of Antwerp, 2610 Antwerp, Belgium.

Vera Pfanzagl (V)

Division of Biochemistry, Department of Chemistry, BOKU-University of Natural Resources and Life Sciences, 1190 Vienna, Austria.

Jeroen Roefs (J)

BIMEF Laboratory, Department of Chemistry, University of Antwerp, 2610 Antwerp, Belgium.

Christian Obinger (C)

Division of Biochemistry, Department of Chemistry, BOKU-University of Natural Resources and Life Sciences, 1190 Vienna, Austria.

Sabine Van Doorslaer (S)

BIMEF Laboratory, Department of Chemistry, University of Antwerp, 2610 Antwerp, Belgium.

Articles similaires

Photosynthesis Ribulose-Bisphosphate Carboxylase Carbon Dioxide Molecular Dynamics Simulation Cyanobacteria
Aspergillus Hydrogen-Ion Concentration Coculture Techniques Secondary Metabolism Streptomyces rimosus

Two codependent routes lead to high-level MRSA.

Abimbola Feyisara Adedeji-Olulana, Katarzyna Wacnik, Lucia Lafage et al.
1.00
Methicillin-Resistant Staphylococcus aureus Penicillin-Binding Proteins Peptidoglycan Bacterial Proteins Anti-Bacterial Agents

Aminoacid functionalised magnetite nanoparticles Fe

Spoială Angela, Motelica Ludmila, Ilie Cornelia-Ioana et al.
1.00
Magnetite Nanoparticles Tryptophan Biocompatible Materials Microbial Sensitivity Tests Humans

Classifications MeSH