Mechanically transduced immunosorbent assay to measure protein-protein interactions.
DIDO1
METRIS
UHRF1
biochemistry
chemical biology
epigenetics
none
physics of living systems
protein-protein interactions
ubiquitin
Journal
eLife
ISSN: 2050-084X
Titre abrégé: Elife
Pays: England
ID NLM: 101579614
Informations de publication
Date de publication:
28 09 2021
28 09 2021
Historique:
received:
18
02
2021
accepted:
28
08
2021
entrez:
28
9
2021
pubmed:
29
9
2021
medline:
9
11
2021
Statut:
epublish
Résumé
Measuring protein-protein interaction (PPI) affinities is fundamental to biochemistry. Yet, conventional methods rely upon the law of mass action and cannot measure many PPIs due to a scarcity of reagents and limitations in the measurable affinity ranges. Here, we present a novel technique that leverages the fundamental concept of friction to produce a mechanical signal that correlates to binding potential. The mechanically transduced immunosorbent (METRIS) assay utilizes rolling magnetic probes to measure PPI interaction affinities. METRIS measures the translational displacement of protein-coated particles on a protein-functionalized substrate. The translational displacement scales with the effective friction induced by a PPI, thus producing a mechanical signal when a binding event occurs. The METRIS assay uses as little as 20 pmols of reagents to measure a wide range of affinities while exhibiting a high resolution and sensitivity. We use METRIS to measure several PPIs that were previously inaccessible using traditional methods, providing new insights into epigenetic recognition.
Identifiants
pubmed: 34581668
doi: 10.7554/eLife.67525
pii: 67525
pmc: PMC8479797
doi:
pii:
Substances chimiques
Immunosorbents
0
Proteins
0
Types de publication
Journal Article
Research Support, N.I.H., Extramural
Research Support, Non-U.S. Gov't
Langues
eng
Sous-ensembles de citation
IM
Subventions
Organisme : NIGMS NIH HHS
ID : R35 GM126900
Pays : United States
Informations de copyright
© 2021, Petell et al.
Déclaration de conflit d'intérêts
CP, KR, MP, DS, BS, JH No competing interests declared, JS is a cofounder of Tribosense Technologies
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