Evaluation of polymerase chain reaction-based open reading frame typing method for the clonality investigation of Clostridioides difficile isolates.

Clostridioides difficile / classification Clostridium Infections / diagnosis Humans Multilocus Sequence Typing Open Reading Frames Phylogeny Polymerase Chain Reaction / methods Reagent Kits, Diagnostic Reproducibility of Results Sensitivity and Specificity
Clostridioides difficile PCR-Based open reading frame typing Simpson's diversity index

Journal

Anaerobe
ISSN: 1095-8274
Titre abrégé: Anaerobe
Pays: England
ID NLM: 9505216

Informations de publication

Date de publication:
Dec 2021
Historique:
received: 15 06 2021
revised: 24 09 2021
accepted: 26 09 2021
pubmed: 1 10 2021
medline: 11 3 2022
entrez: 30 9 2021
Statut: ppublish

Résumé

Here, we assessed the utility of a polymerase chain reaction-based open reading frame typing assay for investigating the clonality of Clostridioides difficile isolates. This assay has a higher discriminatory power than multi-locus sequence typing for molecular epidemiological analysis of C. difficile isolates and can provide additional information about toxin genotypes.

Identifiants

DOI: 10.1016/j.anaerobe.2021.102462 PMID: 34592472
pubmed: 34592472
pii: S1075-9964(21)00145-1
doi: 10.1016/j.anaerobe.2021.102462
pii:
doi:

Substances chimiques

Reagent Kits, Diagnostic 0

Types de publication

Journal Article

Langues

eng

Sous-ensembles de citation

IM

Pagination

102462

Informations de copyright

Copyright © 2021 Elsevier Ltd. All rights reserved.

Déclaration de conflit d'intérêts

Declaration of competing interest None of the authors has any financial relationships with commercial entities that have an interest in the subject of this manuscript.

Auteurs

Yusuke Ota (Y)

Department of Molecular Microbiology, Graduate School of Medicine and Dental Science, Tokyo Medical and Dental University, 1-5-45 Yushima, Bunkyo-ku, Tokyo, 113-8510, Japan. Electronic address: y-ota.micr@tmd.ac.jp.

Natsune Hongou (N)

Department of Molecular Microbiology, Graduate School of Medicine and Dental Science, Tokyo Medical and Dental University, 1-5-45 Yushima, Bunkyo-ku, Tokyo, 113-8510, Japan. Electronic address: 180312ts@tmd.ac.jp.

Yoko Nukui (Y)

Department of Infection Control and Prevention, Tokyo Medical and Dental University Medical Hospital, 1-5-45 Yushima, Bunkyo-ku, Tokyo 113-8510, Japan. Electronic address: y-nukui.infe@tmd.ac.jp.

Ryuji Koike (R)

Clinical Research Center, Tokyo Medical and Dental University Medical Hospital, 1-5-45 Yushima, Bunkyo-ku, Tokyo, 113-8519, Japan. Electronic address: koike.rheu@tmd.ac.jp.

Shuji Tohda (S)

Department of Clinical Laboratory, Tokyo Medical and Dental University, 1-5-45 Yushima, Bunkyo-ku, Tokyo 113-8510, Japan. Electronic address: tohda.mlab@tmd.ac.jp.

Ryoichi Saito (R)

Department of Molecular Microbiology, Graduate School of Medicine and Dental Science, Tokyo Medical and Dental University, 1-5-45 Yushima, Bunkyo-ku, Tokyo, 113-8510, Japan. Electronic address: r-saito.mi@tmd.ac.jp.

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Classifications MeSH

questionsmedicales.fr › Bactéries › Firmicutes › Clostridiales › Clostridioides › Clostridium difficile › Evaluation of polymerase chain reaction-based...
questionsmedicales.fr › Infection › Infections bactériennes et mycoses › Infections bactériennes › Infections bactériennes à Gram positif › Infections à Clostridium › Evaluation of polymerase chain reaction-based...
questionsmedicales.fr › Eucaryotes › Animaux › Chordés › Vertébrés › Mammifères › Eutheria › Primates › Haplorhini › Catarrhini › Hominidae › Humains › Evaluation of polymerase chain reaction-based...
questionsmedicales.fr › Techniques d'investigation › Techniques génétiques › Analyse de séquence › Analyse de séquence d'ADN › Typage par séquençage multilocus › Evaluation of polymerase chain reaction-based...
questionsmedicales.fr › Phénomènes génétiques › Structures génétiques › Génome › Composants de génome › Gènes › Composants de gène › Cadres ouverts de lecture › Evaluation of polymerase chain reaction-based...
questionsmedicales.fr › Sciences de l'information › Phylogenèse › Evaluation of polymerase chain reaction-based...
questionsmedicales.fr › Techniques d'investigation › Techniques génétiques › Techniques d'amplification d'acides nucléiques › Réaction de polymérisation en chaîne › Evaluation of polymerase chain reaction-based...
questionsmedicales.fr › Équipement et fournitures › Trousses de réactifs pour diagnostic › Evaluation of polymerase chain reaction-based...
questionsmedicales.fr › Environnement et santé publique › Santé publique › Méthodes épidémiologiques › Méthodologie en recherche épidémiologique › Reproductibilité des résultats › Evaluation of polymerase chain reaction-based...
questionsmedicales.fr › Environnement et santé publique › Santé publique › Méthodes épidémiologiques › Statistiques comme sujet › Sensibilité et spécificité › Evaluation of polymerase chain reaction-based...