A BTB-TAZ protein is required for gene activation by Cauliflower mosaic virus 35S multimerized enhancers.
Journal
Plant physiology
ISSN: 1532-2548
Titre abrégé: Plant Physiol
Pays: United States
ID NLM: 0401224
Informations de publication
Date de publication:
20 01 2022
20 01 2022
Historique:
received:
07
04
2021
accepted:
27
08
2021
pubmed:
2
10
2021
medline:
11
3
2022
entrez:
1
10
2021
Statut:
ppublish
Résumé
The Arabidopsis (Arabidopsis thaliana) BTB-TAZ DOMAIN PROTEIN 2 (BT2) contains an N-terminal BTB domain, a central TAZ zinc-finger protein-protein interaction domain, and a C-terminal calmodulin-binding domain. We previously demonstrated that BT2 regulates telomerase activity and mediates multiple responses to nutrients, hormones, and abiotic stresses in Arabidopsis. Here, we describe the essential role of BT2 in activation of genes by multimerized Cauliflower mosaic virus 35S (35S) enhancers. Loss of BT2 function in several well-characterized 35S enhancer activation-tagged lines resulted in suppression of the activation phenotypes. Suppression of the phenotypes was associated with decreased transcript abundance of the tagged genes. Nuclear run-on assays, mRNA decay studies, and bisulfite sequencing revealed that BT2 is required to maintain the transcriptionally active state of the multimerized 35S enhancers, and lack of BT2 leads to hypermethylation of the 35S enhancers. The TAZ domain and the Ca++/calmodulin-binding domain of BT2 are critical for its function and 35S enhancer activity. We further demonstrate that BT2 requires CULLIN3 and two bromodomain-containing Global Transcription factor group E proteins (GTE9 and GTE11), to regulate 35S enhancer activity. We propose that the BT2-CULLIN3 ubiquitin ligase, through interactions with GTE9 and GTE11, regulates 35S enhancer activity in Arabidopsis.
Identifiants
pubmed: 34597402
pii: 6375951
doi: 10.1093/plphys/kiab450
pmc: PMC8774732
doi:
Substances chimiques
Plant Proteins
0
Types de publication
Comparative Study
Journal Article
Research Support, Non-U.S. Gov't
Research Support, U.S. Gov't, Non-P.H.S.
Langues
eng
Sous-ensembles de citation
IM
Pagination
397-410Informations de copyright
© The Author(s) 2021. Published by Oxford University Press on behalf of American Society of Plant Biologists.
Références
Plant Physiol. 2011 Dec;157(4):1765-77
pubmed: 21960139
Plant Physiol. 2005 Jan;137(1):83-93
pubmed: 15618422
J Biol Chem. 2005 May 13;280(19):18810-21
pubmed: 15749712
Nature. 2009 Feb 26;457(7233):1154-8
pubmed: 19122675
Plant J. 2009 Apr;58(1):109-21
pubmed: 19054356
Plant Cell. 2003 Aug;15(8):1689-703
pubmed: 12897245
Science. 1990 Nov 16;250(4983):959-66
pubmed: 17746920
Plant Physiol. 2008 May;147(1):41-57
pubmed: 18354039
Nucleic Acids Res. 2012 Jan;40(Database issue):D1202-10
pubmed: 22140109
Plant Physiol. 2016 Jun;171(2):1523-32
pubmed: 27208309
Science. 1992 Nov 20;258(5086):1350-3
pubmed: 1455228
Bioinformatics. 2002 Nov;18(11):1427-31
pubmed: 12424112
J Mol Biol. 2000 Oct 20;303(2):243-53
pubmed: 11023789
Plant Mol Biol. 2004 Mar;54(4):549-69
pubmed: 15316289
Nat Rev Genet. 2014 Jun;15(6):394-408
pubmed: 24805120
Mol Genet Genomics. 2009 Apr;281(4):375-90
pubmed: 19130088
Cell. 2008 May 2;133(3):523-36
pubmed: 18423832
Plant Physiol. 2009 Aug;150(4):1930-9
pubmed: 19525324
Nature. 1985 Feb 28-Mar 6;313(6005):810-2
pubmed: 3974711
Science. 1998 Jul 3;281(5373):60-3
pubmed: 9679020
Proc Natl Acad Sci U S A. 2002 Aug 20;99(17):11513-8
pubmed: 12167669
Genes Dev. 2006 Jul 1;20(13):1790-9
pubmed: 16818609
J Mol Biol. 1992 May 20;225(2):327-48
pubmed: 1317461
Nature. 1989 Aug 31;340(6236):727-30
pubmed: 2528073
Gene. 2008 May 15;414(1-2):67-75
pubmed: 18353569
Plant J. 2002 Mar;29(6):797-808
pubmed: 12148537
Science. 2003 Aug 1;301(5633):653-7
pubmed: 12893945
EMBO J. 1997 Jun 16;16(12):3599-608
pubmed: 9218801
Plant Cell. 2000 Dec;12(12):2383-2394
pubmed: 11148285
Plant Mol Biol. 2018 Mar;96(4-5):393-402
pubmed: 29363002
Plant Mol Biol. 1998 Jul;37(5):885-96
pubmed: 9678583
Plant Cell. 2005 Apr;17(4):1180-95
pubmed: 15772280
Plant Physiol. 2002 Dec;130(4):1636-44
pubmed: 12481047
Genes Dev. 2000 Jul 1;14(13):1553-77
pubmed: 10887150
Plant Mol Biol. 2007 Jun;64(3):329-47
pubmed: 17429742
Plant Physiol. 2008 Jan;146(1):189-99
pubmed: 17993541
EMBO J. 2000 Oct 2;19(19):5194-201
pubmed: 11013221
Development. 1991 Nov;113(3):735-47
pubmed: 1821846
PLoS One. 2016 Mar 16;11(3):e0151323
pubmed: 26982336
Science. 2000 Jul 14;289(5477):295-7
pubmed: 10894776
Nature. 2003 Sep 18;425(6955):257-63
pubmed: 12931144
Plant Cell. 1989 Dec;1(12):1147-56
pubmed: 2535536
Biochem Biophys Res Commun. 2017 Jan 29;483(1):380-386
pubmed: 28025145
Proc Natl Acad Sci U S A. 1987 Jul;84(14):4870-4
pubmed: 16578811
Plant Cell. 2007 Jan;19(1):23-31
pubmed: 17220202
Transgenic Res. 2003 Oct;12(5):615-29
pubmed: 14601660
Plant Physiol. 2000 Apr;122(4):1003-13
pubmed: 10759496
Science. 1983 Feb 11;219(4585):626-31
pubmed: 6297005
Plant J. 2005 Feb;41(3):386-99
pubmed: 15659098
Methods. 2001 Dec;25(4):402-8
pubmed: 11846609
Plant Cell. 1989 Jan;1(1):141-50
pubmed: 2535461
Mol Cell Biol. 1988 Jun;8(6):2555-61
pubmed: 2841583
Curr Biol. 2001 Mar 20;11(6):436-40
pubmed: 11301254
Mol Plant. 2014 Sep;7(9):1494-1496
pubmed: 24719468
Science. 2001 Jan 12;291(5502):306-9
pubmed: 11209081
Nat Methods. 2012 Jul;9(7):671-5
pubmed: 22930834
Nat Protoc. 2006;1(6):3094-100
pubmed: 17406505
J Biol Chem. 1990 Jun 15;265(17):9909-13
pubmed: 2351681
EMBO J. 1999 Jan 4;18(1):241-8
pubmed: 9878066
Nucleic Acids Res. 2008 Jul 1;36(Web Server issue):W170-5
pubmed: 18487274
J Plant Res. 2005 Dec;118(6):391-9
pubmed: 16273423
EMBO J. 1989 Aug;8(8):2195-202
pubmed: 16453896
Plant J. 2014 Jul;79(2):348-59
pubmed: 24836556
Nature. 2009 Oct 22;461(7267):1135-8
pubmed: 19847267
Plant Mol Biol. 2002 Sep;50(1):43-57
pubmed: 12139008
Nucleic Acids Res. 1995 Sep 25;23(18):3778-85
pubmed: 7479010
Plant Cell. 2003 Jun;15(6):1468-79
pubmed: 12782737