Multiplexed Single-Molecule Experiments Reveal Nucleosome Invasion Dynamics of the Cas9 Genome Editor.


Journal

Journal of the American Chemical Society
ISSN: 1520-5126
Titre abrégé: J Am Chem Soc
Pays: United States
ID NLM: 7503056

Informations de publication

Date de publication:
13 10 2021
Historique:
pubmed: 2 10 2021
medline: 19 2 2022
entrez: 1 10 2021
Statut: ppublish

Résumé

Single-molecule measurements provide detailed mechanistic insights into molecular processes, for example in genome regulation where DNA access is controlled by nucleosomes and the chromatin machinery. However, real-time single-molecule observations of nuclear factors acting on defined chromatin substrates are challenging to perform quantitatively and reproducibly. Here we present XSCAN (multiple

Identifiants

pubmed: 34597515
doi: 10.1021/jacs.1c06195
pmc: PMC8517959
doi:

Substances chimiques

Nucleosomes 0

Types de publication

Journal Article Research Support, Non-U.S. Gov't

Langues

eng

Sous-ensembles de citation

IM

Pagination

16313-16319

Subventions

Organisme : Howard Hughes Medical Institute
Pays : United States

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Auteurs

Kristina Makasheva (K)

Laboratory of Biophysical Chemistry of Macromolecules, Institute of Chemical Sciences and Engineering (ISIC), École Polytechnique Fédérale de Lausanne (EPFL), Station 6, 1015 Lausanne, Switzerland.

Louise C Bryan (LC)

Laboratory of Biophysical Chemistry of Macromolecules, Institute of Chemical Sciences and Engineering (ISIC), École Polytechnique Fédérale de Lausanne (EPFL), Station 6, 1015 Lausanne, Switzerland.

Carolin Anders (C)

Department of Biochemistry, University of Zurich, Winterthurerstr. 190, 8057 Zurich, Switzerland.

Sherin Panikulam (S)

Department of Biochemistry, University of Zurich, Winterthurerstr. 190, 8057 Zurich, Switzerland.

Martin Jinek (M)

Department of Biochemistry, University of Zurich, Winterthurerstr. 190, 8057 Zurich, Switzerland.

Beat Fierz (B)

Laboratory of Biophysical Chemistry of Macromolecules, Institute of Chemical Sciences and Engineering (ISIC), École Polytechnique Fédérale de Lausanne (EPFL), Station 6, 1015 Lausanne, Switzerland.

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Classifications MeSH