Effect of ultrasonic treatment on the activity of sugar metabolism relative enzymes and quality of coconut water.
Acid invertase
Coconut water
Inactivation
Sugar metabolism
Ultrasound
Journal
Ultrasonics sonochemistry
ISSN: 1873-2828
Titre abrégé: Ultrason Sonochem
Pays: Netherlands
ID NLM: 9433356
Informations de publication
Date de publication:
Nov 2021
Nov 2021
Historique:
received:
03
08
2021
revised:
14
09
2021
accepted:
01
10
2021
pubmed:
11
10
2021
medline:
5
2
2022
entrez:
10
10
2021
Statut:
ppublish
Résumé
In this study, tender coconuts were treated with high-intensity ultrasound (US) for 20 min at a frequency of 20 kHz and a power of 2400 W. Compared with control group, US treated coconut water had a higher content of total soluble solid and sugar/acid ratio along with a lower pH value and conductivity, and the contents of sucrose, fructose and glucose were also higher. Results from HS-SPME/GC-MS showed that there was no significant difference in the content of volatile compounds in coconut water before and after US treatment. The activities of sugar metabolism enzymes such as sucrose phosphate synthase, sucrose synthase, acid invertase (AI) and neutral invertase were inhibited by US, of which AI had the strongest inactivation. Circular dichroism and fluorescence spectra showed that the secondary and tertiary structure of AI molecule were destroyed with the increase of US intensity and time, which was confirmed by the change of particle size distribution pattern and scanning electron microscopy. Molecular docking and molecular dynamics showed that US treatment prevented the recognition and binding of sucrose and AI molecules, thereby inhibiting the decomposition of sucrose. In conclusion, our results indicate that US can inhibit the activity of AI and maintain the sugar content to increase the quality as well as extend the shelflife of coconut water, which will bring more commercial value.
Identifiants
pubmed: 34628309
pii: S1350-4177(21)00322-9
doi: 10.1016/j.ultsonch.2021.105780
pmc: PMC8501503
pii:
doi:
Substances chimiques
Sugars
0
Sucrose
57-50-1
beta-Fructofuranosidase
EC 3.2.1.26
Types de publication
Journal Article
Langues
eng
Sous-ensembles de citation
IM
Pagination
105780Informations de copyright
Copyright © 2021 The Authors. Published by Elsevier B.V. All rights reserved.
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