The Hda1 histone deacetylase limits divergent non-coding transcription and restricts transcription initiation frequency.
RNA polymerase II transcription
divergent non-coding (DNC) transcription
live-cell imaging
non-coding RNA (ncRNA)
Journal
The EMBO journal
ISSN: 1460-2075
Titre abrégé: EMBO J
Pays: England
ID NLM: 8208664
Informations de publication
Date de publication:
01 12 2021
01 12 2021
Historique:
revised:
31
08
2021
received:
07
06
2021
accepted:
28
09
2021
pubmed:
19
10
2021
medline:
22
12
2021
entrez:
18
10
2021
Statut:
ppublish
Résumé
Nucleosome-depleted regions (NDRs) at gene promoters support initiation of RNA polymerase II transcription. Interestingly, transcription often initiates in both directions, resulting in an mRNA and a divergent non-coding (DNC) transcript of unclear purpose. Here, we characterized the genetic architecture and molecular mechanism of DNC transcription in budding yeast. Using high-throughput reverse genetic screens based on quantitative single-cell fluorescence measurements, we identified the Hda1 histone deacetylase complex (Hda1C) as a repressor of DNC transcription. Nascent transcription profiling showed a genome-wide role of Hda1C in repression of DNC transcription. Live-cell imaging of transcription revealed that mutations in the Hda3 subunit increased the frequency of DNC transcription. Hda1C contributed to decreased acetylation of histone H3 in DNC transcription regions, supporting DNC transcription repression by histone deacetylation. Our data support the interpretation that DNC transcription results as a consequence of the NDR-based architecture of eukaryotic promoters, but that it is governed by locus-specific repression to maintain genome fidelity.
Identifiants
pubmed: 34661296
doi: 10.15252/embj.2021108903
pmc: PMC8634119
doi:
Substances chimiques
Histones
0
Nucleosomes
0
RNA, Untranslated
0
Saccharomyces cerevisiae Proteins
0
RNA Polymerase II
EC 2.7.7.-
HDA1 protein, S cerevisiae
EC 3.5.1.-
Histone Deacetylases
EC 3.5.1.98
Types de publication
Journal Article
Research Support, Non-U.S. Gov't
Langues
eng
Sous-ensembles de citation
IM
Pagination
e108903Informations de copyright
© 2021 The Authors. Published under the terms of the CC BY NC ND 4.0 license.
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