General Tolerance of Galactosyltransferases toward UDP-galactosamine Expands Their Synthetic Capability.
GalNAc-glycosides
UDP-galactosamine
galactosyltransferase
general tolerance
Journal
Angewandte Chemie (International ed. in English)
ISSN: 1521-3773
Titre abrégé: Angew Chem Int Ed Engl
Pays: Germany
ID NLM: 0370543
Informations de publication
Date de publication:
13 12 2021
13 12 2021
Historique:
received:
15
09
2021
pubmed:
19
10
2021
medline:
28
12
2021
entrez:
18
10
2021
Statut:
ppublish
Résumé
Accessing large numbers of structurally diverse glycans and derivatives is essential to functional glycomics. We showed a general tolerance of galactosyltransferases toward uridine-diphosphate-galactosamine (UDP-GalN), which is not a commonly used sugar nucleotide donor. The property was harnessed to develop a two-step chemoenzymatic strategy for facile synthesis of novel and divergent N-acetylgalactosamine (GalNAc)-glycosides and derivatives in preparative scales. The discovery and the application of the new property of existing glycosyltransferases expand their catalytic capabilities in generating novel carbohydrate linkages, thus prompting the synthesis of diverse glycans and glycoconjugates for biological studies.
Identifiants
pubmed: 34661966
doi: 10.1002/anie.202112574
pmc: PMC8720041
mid: NIHMS1751750
doi:
Substances chimiques
uridine diphosphate galactosamine
17479-06-0
Uridine Diphosphate N-Acetylgalactosamine
7277-98-7
Galactosyltransferases
EC 2.4.1.-
Types de publication
Journal Article
Research Support, N.I.H., Extramural
Langues
eng
Sous-ensembles de citation
IM
Pagination
26555-26560Subventions
Organisme : NIGMS NIH HHS
ID : R44 GM123820
Pays : United States
Organisme : NIGMS NIH HHS
ID : U01GM125288
Pays : United States
Organisme : NIGMS NIH HHS
ID : U01 GM125288
Pays : United States
Organisme : NIGMS NIH HHS
ID : R44GM123820
Pays : United States
Organisme : NIA NIH HHS
ID : R56AG062258
Pays : United States
Organisme : NIA NIH HHS
ID : R56 AG062258
Pays : United States
Informations de copyright
© 2021 Wiley-VCH GmbH.
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