High-plex expression profiling reveals that implants drive spatiotemporal protein production and innate immune activation for tissue repair.


Journal

Acta biomaterialia
ISSN: 1878-7568
Titre abrégé: Acta Biomater
Pays: England
ID NLM: 101233144

Informations de publication

Date de publication:
15 01 2022
Historique:
received: 08 06 2021
revised: 08 10 2021
accepted: 12 10 2021
pubmed: 22 10 2021
medline: 28 1 2022
entrez: 21 10 2021
Statut: ppublish

Résumé

Surprisingly little clarity exists concerning effects of biomaterial properties on spatially localized protein expression, which drives implant success. Wound healing and tissue regeneration must be optimally supported by the implant, adsorbed proteins, immune cells, and fibroblasts; cells determine repair and functional recovery through protein production and regulation. However, not yet fully understood is how implants differentially drive spatial quantities of individual proteins both within the implant interior and the tissue surrounding it. Here we apply GeoMx

Identifiants

pubmed: 34673228
pii: S1742-7061(21)00685-1
doi: 10.1016/j.actbio.2021.10.018
pii:
doi:

Substances chimiques

Biocompatible Materials 0

Types de publication

Journal Article Research Support, N.I.H., Extramural Research Support, Non-U.S. Gov't

Langues

eng

Sous-ensembles de citation

IM

Pagination

342-350

Subventions

Organisme : NICHD NIH HHS
ID : R21 HD087828
Pays : United States
Organisme : NIBIB NIH HHS
ID : T32 EB021966
Pays : United States

Informations de copyright

Copyright © 2021 Acta Materialia Inc. Published by Elsevier Ltd. All rights reserved.

Déclaration de conflit d'intérêts

Declaration of Competing Interest PD, JR, and JG are employees and stockholders of NanoString Technologies, Inc.

Auteurs

Prajan Divakar (P)

Thayer School of Engineering, Dartmouth College, Hanover, NH 03755, United States of America; NanoString Technologies, Inc., Seattle, WA, 98109, United States of America.

Jason Reeves (J)

NanoString Technologies, Inc., Seattle, WA, 98109, United States of America.

Jingjing Gong (J)

NanoString Technologies, Inc., Seattle, WA, 98109, United States of America.

Fred W Kolling (FW)

Geisel School of Medicine, Dartmouth College, Hanover, NH, 03755, United States of America.

P Jack Hoopes (P)

Thayer School of Engineering, Dartmouth College, Hanover, NH 03755, United States of America; Geisel School of Medicine, Dartmouth College, Hanover, NH, 03755, United States of America.

Ulrike G K Wegst (UGK)

Thayer School of Engineering, Dartmouth College, Hanover, NH 03755, United States of America; Department of Physics, Northeastern University, Boston, MA, 02115, United States of America. Electronic address: u.wegst@northeastern.edu.

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Classifications MeSH