Genome sequencing of the NIES Cyanobacteria collection with a focus on the heterocyst-forming clade.
Cyanobacteria
culture collection
genome
heterocyst
taxonomy
Journal
DNA research : an international journal for rapid publication of reports on genes and genomes
ISSN: 1756-1663
Titre abrégé: DNA Res
Pays: England
ID NLM: 9423827
Informations de publication
Date de publication:
11 Oct 2021
11 Oct 2021
Historique:
received:
25
09
2021
accepted:
19
10
2021
pubmed:
23
10
2021
medline:
15
12
2021
entrez:
22
10
2021
Statut:
ppublish
Résumé
Cyanobacteria are a diverse group of Gram-negative prokaryotes that perform oxygenic photosynthesis. Cyanobacteria have been used for research on photosynthesis and have attracted attention as a platform for biomaterial/biofuel production. Cyanobacteria are also present in almost all habitats on Earth and have extensive impacts on global ecosystems. Given their biological, economical, and ecological importance, the number of high-quality genome sequences for Cyanobacteria strains is limited. Here, we performed genome sequencing of Cyanobacteria strains in the National Institute for Environmental Studies microbial culture collection in Japan. We sequenced 28 strains that can form a heterocyst, a morphologically distinct cell that is specialized for fixing nitrogen, and 3 non-heterocystous strains. Using Illumina sequencing of paired-end and mate-pair libraries with in silico finishing, we constructed highly contiguous assemblies. We determined the phylogenetic relationship of the sequenced genome assemblies and found potential difficulties in the classification of certain heterocystous clades based on morphological observation. We also revealed a bias on the sequenced strains by the phylogenetic analysis of the 16S rRNA gene including unsequenced strains. Genome sequencing of Cyanobacteria strains deposited in worldwide culture collections will contribute to understanding the enormous genetic and phenotypic diversity within the phylum Cyanobacteria.
Identifiants
pubmed: 34677568
pii: 6408441
doi: 10.1093/dnares/dsab024
pmc: PMC8634303
pii:
doi:
Substances chimiques
RNA, Ribosomal, 16S
0
Types de publication
Journal Article
Langues
eng
Sous-ensembles de citation
IM
Subventions
Organisme : Japan Agency for Medical Research and Development
Organisme : Grant-in-Aid for Scientific Research (C)
ID : 19K06707
Organisme : Japan Society for the Promotion of Science (JSPS)
Informations de copyright
© The Author(s) 2021. Published by Oxford University Press on behalf of Kazusa DNA Research Institute.
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