Assays to Study Enzymatic and Non-Enzymatic Protein Lysine Acetylation In Vitro.

Acetylation Chromatography, Liquid Lysine / metabolism Lysine Acetyltransferases / metabolism Tandem Mass Spectrometry
genetic code expansion lysine acetylation lysine acetyltransferases lysine deacetylases sirtuins

Journal

Current protocols
ISSN: 2691-1299
Titre abrégé: Curr Protoc
Pays: United States
ID NLM: 101773894

Informations de publication

Date de publication:
Nov 2021
Historique:
entrez: 8 11 2021
pubmed: 9 11 2021
medline: 11 11 2021
Statut: ppublish

Résumé

Proteins can be lysine-acetylated both enzymatically, by lysine acetyltransferases (KATs), and non-enzymatically, by acetyl-CoA and/or acetyl-phosphate. Such modification can be reversed by lysine deacetylases classified as NAD

Identifiants

DOI: 10.1002/cpz1.277 PMID: 34748287
pubmed: 34748287
doi: 10.1002/cpz1.277
doi:

Substances chimiques

Lysine Acetyltransferases EC 2.3.1.32
Lysine K3Z4F929H6

Types de publication

Journal Article

Langues

eng

Sous-ensembles de citation

IM

Pagination

e277

Informations de copyright

© 2021 The Authors. Current Protocols published by Wiley Periodicals LLC.

Auteurs

Leonie G Graf (LG)

Department Synthetic and Structural Biochemistry, University of Greifswald, Institute for Biochemistry, Greifswald, Germany.

Robert Vogt (R)

Department Synthetic and Structural Biochemistry, University of Greifswald, Institute for Biochemistry, Greifswald, Germany.

Anna-Theresa Blasl (AT)

Department Synthetic and Structural Biochemistry, University of Greifswald, Institute for Biochemistry, Greifswald, Germany.

Chuan Qin (C)

Department Synthetic and Structural Biochemistry, University of Greifswald, Institute for Biochemistry, Greifswald, Germany.

Sabrina Schulze (S)

Department Synthetic and Structural Biochemistry, University of Greifswald, Institute for Biochemistry, Greifswald, Germany.

Daniela Zühlke (D)

Department of Microbial Physiology and Molecular Biology, University of Greifswald, Institute of Microbiology, Greifswald, Germany.

Susanne Sievers (S)

Department of Microbial Physiology and Molecular Biology, University of Greifswald, Institute of Microbiology, Greifswald, Germany.

Michael Lammers (M)

Department Synthetic and Structural Biochemistry, University of Greifswald, Institute for Biochemistry, Greifswald, Germany.

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Classifications MeSH

questionsmedicales.fr › Métabolisme › Acylation › Acétylation › Assays to Study Enzymatic and Non-Enzymatic...
questionsmedicales.fr › Techniques d'investigation › Techniques de chimie analytique › Chromatographie › Chromatographie en phase liquide › Assays to Study Enzymatic and Non-Enzymatic...
questionsmedicales.fr › Acides aminés, peptides et protéines › Acides aminés › Acides aminés essentiels › Lysine › Assays to Study Enzymatic and Non-Enzymatic...
questionsmedicales.fr › Enzymes et coenzymes › Enzymes › Transferases › Acyltransferases › Acetyltransferases › Lysine acetyltransferases › Assays to Study Enzymatic and Non-Enzymatic...
questionsmedicales.fr › Techniques d'investigation › Techniques de chimie analytique › Spectrométrie de masse › Spectrométrie de masse en tandem › Assays to Study Enzymatic and Non-Enzymatic...