Repression of the Hox gene abd-A by ELAV-mediated Transcriptional Interference.
Animals
Clustered Regularly Interspaced Short Palindromic Repeats
Drosophila Proteins
/ genetics
Drosophila melanogaster
/ embryology
ELAV Proteins
/ genetics
Genes, Homeobox
Genes, Reporter
MicroRNAs
/ genetics
Nuclear Proteins
/ genetics
RNA, Long Noncoding
/ genetics
Sequence Deletion
Transcription Factors
/ genetics
Transcription, Genetic
Journal
PLoS genetics
ISSN: 1553-7404
Titre abrégé: PLoS Genet
Pays: United States
ID NLM: 101239074
Informations de publication
Date de publication:
11 2021
11 2021
Historique:
received:
21
07
2021
accepted:
21
10
2021
revised:
29
11
2021
pubmed:
16
11
2021
medline:
7
1
2022
entrez:
15
11
2021
Statut:
epublish
Résumé
Intergenic transcription is a common feature of eukaryotic genomes and performs important and diverse cellular functions. Here, we investigate the iab-8 ncRNA from the Drosophila Bithorax Complex and show that this RNA is able to repress the transcription of genes located at its 3' end by a sequence-independent, transcriptional interference mechanism. Although this RNA is expressed in the early epidermis and CNS, we find that its repressive activity is limited to the CNS, where, in wild-type embryos, it acts on the Hox gene, abd-A, located immediately downstream of it. The CNS specificity is achieved through a 3' extension of the transcript, mediated by the neuronal-specific, RNA-binding protein, ELAV. Loss of ELAV activity eliminates the 3' extension and results in the ectopic activation of abd-A. Thus, a tissue-specific change in the length of a ncRNA is used to generate a precise pattern of gene expression in a higher eukaryote.
Identifiants
pubmed: 34780465
doi: 10.1371/journal.pgen.1009843
pii: PGENETICS-D-21-00807
pmc: PMC8629391
doi:
Substances chimiques
Drosophila Proteins
0
ELAV Proteins
0
ELAV protein, Drosophila
0
MicroRNAs
0
Nuclear Proteins
0
RNA, Long Noncoding
0
Transcription Factors
0
abd-A protein, Drosophila
132084-67-4
Types de publication
Journal Article
Research Support, Non-U.S. Gov't
Langues
eng
Sous-ensembles de citation
IM
Pagination
e1009843Déclaration de conflit d'intérêts
The authors have declared that no competing interests exist.
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