Grandmaternal cells in cord blood.
Cord blood
HLA compatibility
grandmaternal microchimerism
three generations
Journal
EBioMedicine
ISSN: 2352-3964
Titre abrégé: EBioMedicine
Pays: Netherlands
ID NLM: 101647039
Informations de publication
Date de publication:
Dec 2021
Dec 2021
Historique:
received:
30
08
2021
revised:
09
11
2021
accepted:
15
11
2021
pubmed:
30
11
2021
medline:
22
3
2022
entrez:
29
11
2021
Statut:
ppublish
Résumé
During pregnancy a feto-maternal exchange of cells through the placenta conducts to maternal microchimerism (Mc) in the child and fetal Mc in the mother. Because of this bidirectional traffic of cells, pregnant women have also acquired maternal cells in utero from their mother and could transfer grandmaternal (GdM) cells to their child through the maternal bloodstream during pregnancy. Thus, cord blood (CB) samples could theoretically carry GdMMc. Nevertheless this has never been demonstrated. Using Human Leukocyte Antigen (HLA)-specific quantitative PCR assays on three-generation families, we were able to test 28 CB samples from healthy primigravid women for GdMMc in whole blood (WB) and isolated cells (PBMC, T, B, granulocytes, stem cells). Five CB samples (18%) had GdMMc which could not be confounded with maternal source, with quantities 100 fold lower than maternal Mc in WB and PBMC. Risk of aneuploidies and/or related invasive prenatal procedures significantly correlated with the presence of GdMMc in CB (p=0.024). Significantly decreased HLA compatibility was observed in three-generation families from CB samples carrying GdMMc (p=0.019). Transgenerational transfer of cells could have implications in immunology and evolution. Further analyses will be necessary to evaluate whether GdMMc in CB is a passive or immunologically active transfer and whether invasive prenatal procedures could trigger GdMMc. Provence-Alpes-Côte d'Azur APEX grant # 2012_06549E, 2012_11786F and 2014_03978) and the Foundation for Medical Research (FRM Grant #ING20140129045).
Sections du résumé
BACKGROUND
BACKGROUND
During pregnancy a feto-maternal exchange of cells through the placenta conducts to maternal microchimerism (Mc) in the child and fetal Mc in the mother. Because of this bidirectional traffic of cells, pregnant women have also acquired maternal cells in utero from their mother and could transfer grandmaternal (GdM) cells to their child through the maternal bloodstream during pregnancy. Thus, cord blood (CB) samples could theoretically carry GdMMc. Nevertheless this has never been demonstrated.
METHODS
METHODS
Using Human Leukocyte Antigen (HLA)-specific quantitative PCR assays on three-generation families, we were able to test 28 CB samples from healthy primigravid women for GdMMc in whole blood (WB) and isolated cells (PBMC, T, B, granulocytes, stem cells).
FINDINGS
RESULTS
Five CB samples (18%) had GdMMc which could not be confounded with maternal source, with quantities 100 fold lower than maternal Mc in WB and PBMC. Risk of aneuploidies and/or related invasive prenatal procedures significantly correlated with the presence of GdMMc in CB (p=0.024). Significantly decreased HLA compatibility was observed in three-generation families from CB samples carrying GdMMc (p=0.019).
INTERPRETATION
CONCLUSIONS
Transgenerational transfer of cells could have implications in immunology and evolution. Further analyses will be necessary to evaluate whether GdMMc in CB is a passive or immunologically active transfer and whether invasive prenatal procedures could trigger GdMMc.
FUNDING
BACKGROUND
Provence-Alpes-Côte d'Azur APEX grant # 2012_06549E, 2012_11786F and 2014_03978) and the Foundation for Medical Research (FRM Grant #ING20140129045).
Identifiants
pubmed: 34844192
pii: S2352-3964(21)00515-6
doi: 10.1016/j.ebiom.2021.103721
pmc: PMC8720789
pii:
doi:
Substances chimiques
HLA Antigens
0
Types de publication
Journal Article
Multicenter Study
Langues
eng
Sous-ensembles de citation
IM
Pagination
103721Informations de copyright
Copyright © 2021 The Author(s). Published by Elsevier B.V. All rights reserved.
Déclaration de conflit d'intérêts
Declaration of Competing Interest The authors declare no conflicts of interests.
Références
Cell Transplant. 2019 May;28(5):522-528
pubmed: 29947261
J Clin Invest. 1999 Jul;104(1):41-7
pubmed: 10393697
Front Immunol. 2021 Apr 22;12:651399
pubmed: 33968049
Clin Chem. 2000 Sep;46(9):1301-9
pubmed: 10973858
Lab Invest. 2006 Nov;86(11):1185-92
pubmed: 16969370
Blood. 1995 Oct 1;86(7):2829-32
pubmed: 7545474
Nat Rev Immunol. 2017 Aug;17(8):483-494
pubmed: 28480895
Cell Immunol. 2007 Feb;245(2):80-90
pubmed: 17524378
Chimerism. 2015 Oct 2;6(4):65-71
pubmed: 27623703
BMJ. 1996 Dec 7;313(7070):1445-9
pubmed: 8973233
Bone Marrow Transplant. 2021 May;56(5):1090-1098
pubmed: 33257776
Chimerism. 2012 Jul-Dec;3(3):1-8
pubmed: 22926759
Med J Aust. 2006 Apr 17;184(8):407-10
pubmed: 16618241
PLoS One. 2011;6(8):e24101
pubmed: 21912617
Clin Exp Allergy. 2018 Feb;48(2):167-174
pubmed: 28925522
Semin Cell Dev Biol. 2015 Jul;43:131-140
pubmed: 25937492
J Hematother. 1993 Summer;2(2):235-9
pubmed: 7921983
Oncoimmunology. 2017 Mar 31;6(5):e1311436
pubmed: 28638735
Arthritis Rheum. 2009 Jan;60(1):73-80
pubmed: 19117368
Am J Obstet Gynecol. 1964 Mar 1;88:565-71
pubmed: 14128187
Sci Rep. 2015 Sep 29;5:14466
pubmed: 26417717
J Matern Fetal Neonatal Med. 2017 Nov;30(21):2613-2619
pubmed: 27923274
BJOG. 2000 Oct;107(10):1265-70
pubmed: 11028579
Sci Rep. 2019 Mar 27;9(1):5247
pubmed: 30918307
Bone Marrow Transplant. 1998 Jun;21(11):1097-9
pubmed: 9645571
Exp Hematol. 1995 Dec;23(14):1601-5
pubmed: 8542953