Development of simplex and multiplex RT-qPCR assays for the detection of three cryptic viruses of black-grass (Alopecurus myosuroides).
AMPV1
AMPV2
AMVV1
Alopecurus myosuroides
Quantification
RT-qPCR
Journal
Journal of virological methods
ISSN: 1879-0984
Titre abrégé: J Virol Methods
Pays: Netherlands
ID NLM: 8005839
Informations de publication
Date de publication:
02 2022
02 2022
Historique:
received:
09
03
2021
revised:
17
06
2021
accepted:
26
11
2021
pubmed:
2
12
2021
medline:
22
3
2022
entrez:
1
12
2021
Statut:
ppublish
Résumé
Simplex and multiplex RT-qPCR assays were developed for Alopecurus myosuroides partitivirus 1 (AMPV1), Alopecurus myosuroides partitivirus 2 (AMPV2) and Alopecurus myosuroides varicosavirus 1 (AMVV1), and compared to the existing conventional PCR assays. All assays had a high specificity and their sensitivity was increased compared to the conventional RT-PCR assays. As viral quantification is an important element in comparative experiments, the effect of high- and low-temperature drying treatments, prior to RNA extraction and analysis, was studied and optimised. AMVV1 detection was reduced by both drying treatments, but particularly by the high-temperature. AMPV1 and AMPV2 detection on the other hand was not impeded by the drying treatments, and enables standardisation of plant tissue prior to extraction, in particular for quantitative analysis.
Identifiants
pubmed: 34848282
pii: S0166-0934(21)00328-1
doi: 10.1016/j.jviromet.2021.114389
pii:
doi:
Substances chimiques
Herbicides
0
Types de publication
Journal Article
Research Support, Non-U.S. Gov't
Langues
eng
Sous-ensembles de citation
IM
Pagination
114389Informations de copyright
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