Auxin biosynthesis maintains embryo identity and growth during BABY BOOM-induced somatic embryogenesis.
Journal
Plant physiology
ISSN: 1532-2548
Titre abrégé: Plant Physiol
Pays: United States
ID NLM: 0401224
Informations de publication
Date de publication:
04 02 2022
04 02 2022
Historique:
received:
10
08
2021
accepted:
03
11
2021
pubmed:
6
12
2021
medline:
19
3
2022
entrez:
5
12
2021
Statut:
ppublish
Résumé
Somatic embryogenesis is a type of plant cell totipotency where embryos develop from nonreproductive (vegetative) cells without fertilization. Somatic embryogenesis can be induced in vitro by auxins, and by ectopic expression of embryo-expressed transcription factors like the BABY BOOM (BBM) AINTEGUMENTA-LIKE APETALA2/ETHYLENE RESPONSE FACTOR domain protein. These different pathways are thought to converge to promote auxin response and biosynthesis, but the specific roles of the endogenous auxin pathway in somatic embryogenesis induction have not been well-characterized. Here we show that BBM transcriptionally regulates the YUCCA3 (YUC3) and YUC8 auxin biosynthesis genes during BBM-mediated somatic embryogenesis in Arabidopsis (Arabidopsis thaliana) seedlings. BBM induced local and ectopic YUC3 and YUC8 expression in seedlings, which coincided with increased DR5 auxin response and indole-3-acetic acid (IAA) biosynthesis and with ectopic expression of the WOX2 embryo reporter. YUC-driven auxin biosynthesis was required for BBM-mediated somatic embryogenesis, as the number of embryogenic explants was reduced by ca. 50% in yuc3 yuc8 mutants and abolished after chemical inhibition of YUC enzyme activity. However, a detailed YUC inhibitor time-course study revealed that YUC-dependent IAA biosynthesis is not required for the re-initiation of totipotent cell identity in seedlings. Rather, YUC enzymes are required later in somatic embryo development for the maintenance of embryo identity and growth. This study resolves a long-standing question about the role of endogenous auxin biosynthesis in transcription factor-mediated somatic embryogenesis and also provides an experimental framework for understanding the role of endogenous auxin biosynthesis in other in planta and in vitro embryogenesis systems.
Identifiants
pubmed: 34865162
pii: 6447520
doi: 10.1093/plphys/kiab558
pmc: PMC8825264
doi:
Substances chimiques
Indoleacetic Acids
0
Plant Growth Regulators
0
Transcription Factors
0
Types de publication
Comparative Study
Journal Article
Research Support, Non-U.S. Gov't
Langues
eng
Sous-ensembles de citation
IM
Pagination
1095-1110Informations de copyright
© The Author(s) 2021. Published by Oxford University Press on behalf of American Society of Plant Biologists.
Références
Plants (Basel). 2020 Mar 10;9(3):
pubmed: 32164149
Proc Natl Acad Sci U S A. 2015 Apr 14;112(15):4821-6
pubmed: 25831515
Genes (Basel). 2020 Mar 20;11(3):
pubmed: 32245021
Plant J. 2012 Aug;71(3):427-42
pubmed: 22429691
Mol Plant. 2013 Jul;6(4):1247-60
pubmed: 23271028
Stem Cells Dev. 2014 Apr 15;23(8):796-812
pubmed: 24368070
J Ultrastruct Res. 1967 May;18(3):428-43
pubmed: 6025110
Plant Physiol. 2002 Aug;129(4):1807-19
pubmed: 12177494
Planta. 2011 May;233(5):873-81
pubmed: 21225281
Plant Mol Biol. 2012 Apr;78(6):545-59
pubmed: 22318676
Arabidopsis Book. 2014 Jun 13;12:e0173
pubmed: 24955076
Plant Cell. 2007 Aug;19(8):2430-9
pubmed: 17704214
Nature. 2007 Oct 25;449(7165):1053-7
pubmed: 17960244
Nat Plants. 2018 Aug;4(8):548-553
pubmed: 30013211
Planta. 1997;202(3):387-96
pubmed: 9232908
Proc Natl Acad Sci U S A. 2016 Sep 27;113(39):10742-4
pubmed: 27651487
Trends Plant Sci. 2007 Jun;12(6):245-52
pubmed: 17499544
Nature. 2017 Nov 2;551(7678):124-128
pubmed: 29072296
Planta. 2007 Aug;226(3):619-28
pubmed: 17406890
Plant J. 1998 Dec;16(6):735-43
pubmed: 10069079
Planta. 2013 Sep;238(3):425-40
pubmed: 23722561
New Phytol. 2017 Mar;213(4):1740-1754
pubmed: 27878992
Regeneration (Oxf). 2017 Dec 05;4(4):201-216
pubmed: 29299323
Proc Natl Acad Sci U S A. 2007 Sep 25;104(39):15578-83
pubmed: 17893341
PLoS One. 2011 Feb 18;6(2):e16765
pubmed: 21364738
J Exp Bot. 2020 May 9;71(9):2612-2628
pubmed: 31974549
Proc Natl Acad Sci U S A. 2008 Feb 26;105(8):3151-6
pubmed: 18287041
Plant Cell. 2016 Dec;28(12):2937-2951
pubmed: 27920338
Plant J. 2013 May;74(4):626-37
pubmed: 23425284
Development. 2015 Feb 1;142(3):454-64
pubmed: 25564655
Plant Cell. 2015 Nov;27(11):3099-111
pubmed: 26566918
PLoS One. 2019 Jan 9;14(1):e0204778
pubmed: 30625150
Front Plant Sci. 2019 Apr 26;10:536
pubmed: 31134106
Int J Mol Sci. 2020 Jul 03;21(13):
pubmed: 32635392
Nat Commun. 2021 May 4;12(1):2508
pubmed: 33947865
Methods Mol Biol. 2011;710:257-65
pubmed: 21207274
Plant Physiol. 2017 Oct;175(2):848-857
pubmed: 28830937
Plant J. 2014 Feb;77(3):352-66
pubmed: 24299123
Biochem J. 2020 Oct 16;477(19):3743-3767
pubmed: 33045058
Genes Dev. 2006 Jul 1;20(13):1790-9
pubmed: 16818609
Cell. 2003 Nov 26;115(5):591-602
pubmed: 14651850
Plant J. 2009 Aug;59(3):448-60
pubmed: 19453451
Plant J. 2003 Apr;34(1):107-14
pubmed: 12662313
Nature. 2001 Jul 5;412(6842):39
pubmed: 11452293
Planta. 2005 Dec;222(6):977-88
pubmed: 16034595
Plants (Basel). 2019 Feb 11;8(2):
pubmed: 30754699
Int J Mol Sci. 2020 Feb 16;21(4):
pubmed: 32079138
Proc Natl Acad Sci U S A. 2013 Jan 15;110(3):1107-12
pubmed: 23277580
Plant Cell. 2011 Nov;23(11):3944-60
pubmed: 22108404
Sci Rep. 2019 Feb 8;9(1):1696
pubmed: 30737425
Front Plant Sci. 2019 Feb 19;10:128
pubmed: 30838007
Plant Cell. 2014 Jun 20;26(6):2568-2581
pubmed: 24951481
Dev Cell. 2020 Sep 28;54(6):742-757.e8
pubmed: 32755547
Plant Cell. 2007 Jul;19(7):2197-212
pubmed: 17630274
Plant Cell. 2021 Jul 19;33(6):1907-1926
pubmed: 33730150
Cell. 2008 Apr 4;133(1):177-91
pubmed: 18394997
Plant Physiol. 2011 Dec;157(4):1805-19
pubmed: 22003085
Methods. 2001 Dec;25(4):402-8
pubmed: 11846609
Nature. 2003 Nov 13;426(6963):147-53
pubmed: 14614497
Electrophoresis. 2000 Oct;21(16):3488-99
pubmed: 11079568
Cold Spring Harb Perspect Biol. 2010 Jan;2(1):a001594
pubmed: 20182605
Plant Cell Rep. 2009 Nov;28(11):1677-88
pubmed: 19763577
Plant Physiol. 2011 Feb;155(2):721-34
pubmed: 21119044
Proc Natl Acad Sci U S A. 2001 Sep 25;98(20):11806-11
pubmed: 11573014
Cell. 1998 Jun 26;93(7):1195-205
pubmed: 9657152
Plant Mol Biol. 2008 Oct;68(3):225-37
pubmed: 18663586
Plant Cell. 2011 Nov;23(11):3961-73
pubmed: 22108406
Dev Cell. 2008 Jun;14(6):867-76
pubmed: 18539115
Plant Cell Physiol. 2014 Jun;55(6):1072-9
pubmed: 24562917
Curr Biol. 2013 Dec 16;23(24):2506-12
pubmed: 24291089
Plant Physiol. 2005 Sep;139(1):5-17
pubmed: 16166256
Front Plant Sci. 2015 Aug 18;6:635
pubmed: 26347757
Nat Methods. 2015 Mar;12(3):207-10, 2 p following 210
pubmed: 25643149
Sci Rep. 2017 Oct 25;7(1):13992
pubmed: 29070794
Plant J. 2014 Jul;79(2):348-59
pubmed: 24836556
Plant Cell. 1997 Mar;9(3):355-65
pubmed: 9090880