RNA modifications detection by comparative Nanopore direct RNA sequencing.
Journal
Nature communications
ISSN: 2041-1723
Titre abrégé: Nat Commun
Pays: England
ID NLM: 101528555
Informations de publication
Date de publication:
10 12 2021
10 12 2021
Historique:
received:
31
08
2021
accepted:
09
11
2021
entrez:
11
12
2021
pubmed:
12
12
2021
medline:
11
1
2022
Statut:
epublish
Résumé
RNA molecules undergo a vast array of chemical post-transcriptional modifications (PTMs) that can affect their structure and interaction properties. In recent years, a growing number of PTMs have been successfully mapped to the transcriptome using experimental approaches relying on high-throughput sequencing. Oxford Nanopore direct-RNA sequencing has been shown to be sensitive to RNA modifications. We developed and validated Nanocompore, a robust analytical framework that identifies modifications from these data. Our strategy compares an RNA sample of interest against a non-modified control sample, not requiring a training set and allowing the use of replicates. We show that Nanocompore can detect different RNA modifications with position accuracy in vitro, and we apply it to profile m
Identifiants
pubmed: 34893601
doi: 10.1038/s41467-021-27393-3
pii: 10.1038/s41467-021-27393-3
pmc: PMC8664944
doi:
Substances chimiques
RNA
63231-63-0
Types de publication
Journal Article
Research Support, Non-U.S. Gov't
Langues
eng
Sous-ensembles de citation
IM
Pagination
7198Subventions
Organisme : Wellcome Trust
ID : FC001203
Pays : United Kingdom
Organisme : Cancer Research UK
ID : 12475
Pays : United Kingdom
Organisme : Cancer Research UK
ID : C6946/A24843
Pays : United Kingdom
Organisme : Arthritis Research UK
ID : FC001203
Pays : United Kingdom
Organisme : Cancer Research UK
ID : 10827
Pays : United Kingdom
Organisme : Medical Research Council
Pays : United Kingdom
Organisme : Wellcome Trust (Wellcome)
ID : WT203144
Organisme : Biotechnology and Biological Sciences Research Council
ID : BB/K017438/1
Pays : United Kingdom
Organisme : Wellcome Trust
Pays : United Kingdom
Organisme : Cancer Research UK
ID : RG72100
Pays : United Kingdom
Informations de copyright
© 2021. The Author(s).
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