Labeling of DNA Probes by Polymerase Chain Reaction.

DNA / genetics DNA Probes Polymerase Chain Reaction / methods

Journal

Cold Spring Harbor protocols
ISSN: 1559-6095
Titre abrégé: Cold Spring Harb Protoc
Pays: United States
ID NLM: 101524530

Informations de publication

Date de publication:
01 03 2022
Historique:
pubmed: 16 12 2021
medline: 23 4 2022
entrez: 15 12 2021
Statut: epublish

Résumé

The polymerase chain reaction (PCR) can be used to produce both nonradiolabeled DNA probes and radiolabeled DNA probes with high specific activity. In this protocol, PCR is used to generate double-stranded probes. Related methods, including the generation of asymmetric probes by PCR, are also discussed.

Identifiants

DOI: 10.1101/pdb.prot100610 PMID: 34907078
pubmed: 34907078
pii: pdb.prot100610
doi: 10.1101/pdb.prot100610
doi:

Substances chimiques

DNA Probes 0
DNA 9007-49-2

Types de publication

Journal Article

Langues

eng

Sous-ensembles de citation

IM

Informations de copyright

© 2022 Cold Spring Harbor Laboratory Press.

Auteurs

Michael R Green (MR)

Joseph Sambrook (J)

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Classifications MeSH

questionsmedicales.fr › Acides nucléiques, nucléotides et nucléosides › Acides nucléiques › ADN › Labeling of DNA Probes by Polymerase Chain Reaction.
questionsmedicales.fr › Actions chimiques et utilisations › Utilisations spécialisées de produits chimiques › Produits chimiques de laboratoire › Sondes moléculaires › Sondes d'acide nucléique › Sondes d'ADN › Labeling of DNA Probes by Polymerase Chain Reaction.
questionsmedicales.fr › Techniques d'investigation › Techniques génétiques › Techniques d'amplification d'acides nucléiques › Réaction de polymérisation en chaîne › Labeling of DNA Probes by Polymerase Chain Reaction.